الفهرس 
Review of LiteratureOnly 14 pages are availabe for public view
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Abstract
Multiple sclerosis is a major health problem affecting young adults. It is an inflammatory demyelinating and neurodegenerative disease whose incidence and prevalence are increasing worldwide. It is a multifactorial disease influenced by both genetic and environmental factors resulting in an immune-mediated injury of the CNS. The diagnosis depends mainly on clinical picture, MRI findings and CSF oligoclonal bands.
The field of circulating biological markers showed a great potential in the clinical settings. It could be used as diagnostic, predictive, and prognostic markers and they would open doors to new ways of monitoring the progression of disease and response to treatment. Circulating microRNAs are very stable in plasma and serum, this is the reason why many studies extensively assessed their potential as an ideal immune biomarker in MS and many other diseases.
This study aimed at evaluating miRNAs; hsa-miR-454 and hsa-miR-92a-1* as diagnostic tools among MS Egyptian patients and correlating their association with disease severity in terms of EDSS.
Thirty-one MS patients with 21 RRMS patients and 10 PMS patients fulfilling the McDonald criteria for diagnosis and classification of MS were included in the study in addition to 20 healthy subjects. Patient receiving disease modifying drugs or steroids and patients with other neurological conditions affecting CNS were excluded. Quantitation of plasma hsa-miR-454 and hsa-miR-92a-1* by real-time quantitative polymerase chain reaction was done after miRNA extraction and cDNA production via reverse transcription.
Our results showed that circulating plasma microRNAs hsa-miR-454 and hsa-miR-92a-1* relative expression didn’t differentiate patients from controls. However, their relative expression was significantly higher among patients in relapse in comparison to patients in remission. Additionally, circulating hsa-miR-454 relative expression differentiated RRMS patients from PMS patients.
In conclusion, the results of the current study indicated that circulating hsa-miR-454 and hsa-miR-92a-1* profiles in MS can serve as non-invasive biomarkers of disease activity and MS type discrimination and this shows their promising potential as diagnostic and prognostic tools in MS patients.