الفهرس 
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Abstract
Osteoarthritis is the most prevalent form of arthritis in adults associated with high socioeconomic burden and is a major cause of disability in elderly.
Regenerative medicine based on stem cells is a considered a promising treatment modality for many orthopedic problems. Mesenchymal stem cells (MSCs) from different sources have been extensively investigated for their capability to regenerate damaged or injured articular cartilage and to delay the progression of knee OA. Stromal vascular fraction (SVF) contains cells population with multi-lineage potential and can be easily obtained in large amounts from lipoaspirates. It shares the same properties as adipose derived stem cells ADSCs but doesn’t need to be cultured.
This current study was designed to study the therapeutic role of adipose derived SVF in rat model of collagenase induced osteoarthritis. Osteorthritis was induced in right knee joints of 42 female Wistar rats. The left knee was considered as healthy control.
In our experiment, SVF was obtained by enzymatic degradation of fat obtained from the 6 male rats and yielded SVF 106 cells /ml. Flow cytometry analysis showed that SVF cells were positive for mesenchymal stem cells markers (CD44).
In this work, the treatment groups were injected once with SVF at the 14th day after induction of arthritis. Osteoarthritic clinical and histopathological changes in joint started 2 weeks post induction of osteoarthritis.
At 4 weeks and 8 weeks after induction of osteoarthritis is proven, there was very highly significant difference between groups treated with SVF, induced osteoarthritis and healthy controls regarding modified Mankin score for cartilage degeneration, it was highest in OA untreated group (group II), much less in SVF treatment group (group III) and least in healthy control group(group I), P<0.001. Moreover, there was significant difference between induced osteoarthritis group, treatment group and healthy controls regarding articular cartilage thickness, chondrocyte number and even matrix stain using Hx&E and safranin O stain calculated using image analysis where P<0.001.
In our work the significant difference observed between treatment group and untreated OA regarding modified Mankin Score at 1 month mean ±SD (10.75 ± 0.5, 2.50 ± 0.5, P<0.001) and 2 months (8.50 ± 0.6, 0.50 ± 0.53, P<0.001) respectively denotes decreased progression of osteoarthritis and cartilage degeneration in treatment group. The significant decrease in articular cartilage thickness in untreated OA group compared to SVF treated groups quantified using image analysis both at 1 and 2 months (P<0.05, P=0.001) respectively.
Also, the significant difference between the two groups regarding cell count which was much reduced in untreated induced arthritis group at 1 and 2 months (20.75 ± 1.71 and 26.25 ± 3.30 cells/ HPF) respectively compared to those treated with SVF (42.10 ± 3.18 and 39.40 ± 2.88 cells/HPF), P<0.001. In addition, the difference between groups in Safranin O stain (P<0.001) which reflect difference in proteoglycan content. These findings support the regenerative role of SVF.