الفهرس 
IntroductionOnly 14 pages are availabe for public view
Abstract
Gliding Arc Discharge (GAD) non thermal plasma is one type of important new technology in the field of biomedical applications, such as skin disease, wound healing and cancer treatment. This low temperature atmospheric-pressure plasma is a multi-component system that includes biologically active agents, charged particles, reactive nitrogen and oxygen species, metastable-state molecules or atoms, and UV radiation. The main objective of this study is to investigate the effect of Gliding Arc Discharge on Acute Myelogenous leukemia cell line (AML). Different doses depends on time of exposure (40, 60 and 80 sec) applied for one time, the cells going for culture 72 hours. Two types of analysis were carried out. The first one is 3- (4, 5-dimethyl thiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay where the viability of cell showed significant decrease in all treated samples, also its realised that increasing the time of exposure leads to a good treatment. the second analysis was molecular proteomics assay for two genes which are caspase-3 and cox-2 gene. It was found that, exposure for non thermal plasma gliding arc increase the level of gene transcription(protein expression)of caspase-3 gene (Apoptotic gene) which lead to more degradation of malignant cells, where the transcription of cox-2 gene (inflammatory gene) were reduced.
Conclusion: The gliding Arc non thermal plasma have a highly toxic effect on leukemia (blood cancer), and mechanism of action coming from, the chemical and physical component of the plasma on transcription of apoptotic genes and tumorgenesis genes.