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العنوان
Phytochemical, Biological, Total
Phenol, Antioxidant And HPLC Studies
Of Some Medicinal Plants in Libya /
المؤلف
Kahal, Fatma Saad Ehmed.
هيئة الاعداد
باحث / فاطمة سعد احميد كحل
مشرف / مني عبد العزيز احمد
مشرف / عصام عبد العزيزكيشار
مشرف / سالم محمد ادراه
تاريخ النشر
2021.
عدد الصفحات
128 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Inorganic Chemistry
تاريخ الإجازة
1/1/2021
مكان الإجازة
جامعة عين شمس - كلية العلوم - قسم الكيمياء
الفهرس
Only 14 pages are availabe for public view

Abstract

This study involves phytochemical screening, Antibacterial Activity, antioxidant, total phenolic content, HPLC of the Phenolic Acids and HPLC of the sugar, for the medicinal indigenous plants from Libya (Ruta Chalepensis, Lycium shawii, Cymbopogan Citratus, Euphorbia lathyris and Plantago major leaves). Leaves extracts of the selected medicinal plants
Phytochemical screening: The results of phytochemical screening of methanolic and chloroform extracts obviously show that presence of each tannin, phenols, flavonoids, alkaloids, saponins, cardiac glycosides and carbohydrates,
Antibacterial Activity: Through Disk Diffusion Method, extracts were examined against the activity of Gram-negative pathogenic bacteria Escherichia coli, where, the methanolic extracts revealed a good inhibition effect result, Whereas chloroform extracts revealed a vulnerable inhibition effect result against Escherichia coli,
Antioxidant Activity: Antioxidant potential of the extracts was analyzed as contents of total phenols and free radical scavenging activity extract were assessed by the declaration of a methanolic solution of 2, 2- diphenyl-1-picrylhydrazyl DPPH. Results of antioxidant activities were determined in three concentrations: 50mg/ml, 100 and 200 mg/ml of each extract. And showed that at increasing the concentration, the susceptibility extracts tending to increase also for DPPH, whereas sample Cymbopogan Citratus was the highest concentration is 200 ml, e sample. And at the concentration 200 ml was a sample of Cymbopogan Citratus.
Total Phenolic compounds: The phenolic content was determined by using Folin-Ciocalteau assay, the results obtained showed for methanolic extracts that 3.164, 3.385, 16.737, 0.529 and 6.142 mg/g, was for each plant
HPLC Analysis:
a) HPLC analyses for the quantitive of phenol’s compounds present in the extracts of selected medicinal plants leaves: The HPLC analysis of the plant’s extracts revealed the presence of phenolic compounds which are Caffeic, Vanillin, Ellagic, Ferulic and Rutin found in different concentrations of the plant leaves extracts. Used the SPSS program exhibited a strong significant positive relationship, between Antioxidant and concentration of all plants leaves extracts.
B) HPLC analyses for the quantitive of sugar’s compounds present in the extracts of selected medicinal plants leaves:
The HPLC analysis of the plant’s extracts revealed the presence of sugar’s compounds as the following: Ruta Chalepensis contained Glucose and Galactose, Lycium shawii contained Fructose, Cymbopogan Citratus contained Sucrose and Glucose, Euphorbia lathyris contained Maltose, Glucose, Fructose, and Plantago major contained Sucrose, Glucose, Galactose were found in different concentrations in the plant leaves extracts.