الفهرس 
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Abstract
Ovarian cancer is considered the deadliest gynecologic malignancy among women cancers. It’s classified the 7th most common cancer in women world-wide and the 3rd most common female cancer after breast and cervix cancer, with 22.530 estimated cases and 13,940 death cases. In Egypt, ovarian cancer represents 4.1% of all new cases. There are numerous risk factors involved in the incidence of ovarian cancer which classified into genetic and non-genetic factors; in addition there are protective factors such as pregnancy and tubal ligation. Ovarian cancer called the silent killer as the signs and symptoms are not detected except in late stages. The available options for ovarian cancer treatments whether surgical or therapeutic drugs options are fraught with restrictions and side effects that reflect on the desired results in addition to the high cost, so it was necessary to find a suitable solution to overcome these obstacles. Selenium is an essential macronutrient isolated from chalcopyrite which obtained from the diet or nutritional supplement. Organoselenium compounds have distinctive mechanisms of action as anti-microbial, anti-oxidant, anti-inflammatory and anti-cancerous activities. Herein, we focus on the possible mechanisms that may underlie the anti-cancer activities of organoselenium compounds on sensitive (A2780), cis-platin resistant (A2780CP), and SKOV-3 ovarian cancer cells. The cytotoxicity of forty two organoselenium compounds against ovarian cancer cell lines (A2780, A2780CP, and SKOV-3) was tested. The most active compound was investigated for its ability to inhibit cell migration and metastases using wound healing, colony assay, and determination the activity of MMP-2 and MMP-9 by zymography assay. This compound was further investigated for its ability to induce apoptosis through inducing the production of ROS which lead to DNA fragmentation which detected by comet assay. Flow cytometric analysis was evaluated to study the efficacy of this anti-cancer compound on cell differentiation and possible cell phase at which it induces apoptosis and possible mechanism of action. Western blot assay was made to evaluate the ability of compound to decrease the expression level of PI3K, AKT, and m-TOR proteins which responsible for cell cycle regulation, proliferation, and apoptosis.