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العنوان
Impact of dry orange (Citrus sinensis) peels powder on Bisphenol A induced-hepatic and splenic toxicity in rats /
المؤلف
Abd-Elgwaad, Hager Mosaad Saad.
هيئة الاعداد
باحث / هاجر مسعد سعد عبدالجواد
مشرف / حنان محمد فتحي عبدالوهاب
مشرف / إيناس على كامل
مشرف / إيمان حسن عبدالعزيز
تاريخ النشر
2020.
عدد الصفحات
258 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Biochemistry
تاريخ الإجازة
6/10/2020
مكان الإجازة
جامعة عين شمس - كلية العلوم - كيمياء حيوية
الفهرس
Only 14 pages are availabe for public view

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from 258

Abstract

This study was conducted to investigate the protective effect of orange peels powder (OPP) dietary supplementation on rats orally administered BPA for 4 weeks.
Data revealed that each 100g of OPP contain 5 g protein, 5.61 g fat, 65.67 g carbohydrate, 2.9 g ash, 8.5g moisture and 12.22 g fiber. OPP ethanolic extract had been analyzed for its total phenols and flavonoids content. The results showed that, total phenolic content was 567.93 mg as gallic acid equivalent (GAE), while flavonoids were
162.12 mg as quercetin equivalent (QE)/per 100 g orange peel powder.
In this study, number of 60 male albino rats weighing 130±10g were divided into 6 groups, 10 rats for each as follows:
group 1: (negative control): Rats were fed on balanced diet and received corn oil.
group 2: (positive control or BPA group): Rats were fed on balanced diet + orally administered BPA suspended in corn oil (350mg/kg b.w. twice weekly).
group 3: Rats were fed on balanced diet supplemented with OPP (12.5g/kg diet) + BPA.
group 4: Rats were fed on balanced diet supplemented with OPP (25g/kg diet) + BPA.
group 5: Rats were fed on balanced diet supplemented with OPP (50/kg diet) + BPA.
group 6: Rats were fed on balanced diet supplemented with OPP (100g/kg diet) + BPA.
At the end of the experimental period, the following parameters were determined:
- Change in body weight, food intake, FER and relative weight of liver and spleen.
- Liver SREBF1 gene expression and catalase activity.
- Serum NO level, ALT and AST activities, TC, TAGs, HDL-C, LDL-C, VLDL-C, risk factor1, IL-4, IgM, IgE,
iron and TIBC levels.
-Hematological parameters including Hb%, RBCs count and TLC were analyzed.
- Histopathological examination was carried out for hepatic and splenic sections for all experimental groups to confirm the biochemical results.
The presented study revealed the following results:
1- The biological data reported that, there were no significant differences (P<0.05) in body weight changes, food intake, feed efficiency ratio (FER) and the relative weight of liver and spleen in the experimental groups supplemented with OPP (G3, G4, G5 & G6) as compared to either -ve control group or BPA group(P<0.05).
2- The oral administration of BPA led to a significant increase of liver SREBF1 gene expression by 374% as compared with -ve control group, while there was a significant decrease of liver SREBF1 expression in (G3, G4,
G5 & G6) supplemented with (1.25, 2.5, 5&10g) OPP/kg diet, respectively.
3- It was observed that serum interluken-4 level was significantly increased in BPA group by 285.37 % as compared with -ve control group. On the other hand, there was a significant decrease in serum IL-4 level in OPP supplemented groups (G3, G4, G5 & G6) when compared with BPA group. The most significant decrease was evident in G6 (100 g OPP/kg diet) as compared to G3, G4 and G5.
4- Serum IgM was significantly decreased in BPA group after oral administration of BPA when compared with -ve control group. While OPP supplementation led to a significant increase of serum IgM in G3, G4, G5 and G6 as compared with BPA group. Results also showed that the most significant increase in serum IgM was recorded in G6 as compared by other OPP received groups.
5- On the other hand, findings showed that, despite the significant elevation of serum IgE in BPA group compared with -ve control group, there was a significant decrement in OPP supplemented groups as compared with BPA group. It was observed that, the improvement in serum IgE levels was in a dose dependent manner as, G6 showed the lowest significant decrease compared to other treated groups.
6- There was a significant increment in serum nitric oxide level in BPA group by 538.25% when compared with -ve control group. Also, activities of ALT and AST in serum of BPA group also elevated when compared with -ve control group. On the other hand, all these
parameters were significantly decreased in G3, G4, G5 and G6 which were supplemented by OPP (12.5, 25, 50& 100g/kg diet).
7- The results revealed that, BPA administration caused a significant reduction in liver catalase activity in BPA group by -27.12% as compared to -ve control group. While diet supplementation with OPP significantly increased liver catalase activity.
8- Oral administration of BPA led to significant elevation in serum TC, TAGs, LDL-C, VLDL-C levels and rick factor1 with concomitant reduction of HDL-C in comparison with -ve control group (p<0.05). While rat groups supplemented with OPP showed a significant improvement in these altered lipids profile in a dose dependent manner. This improvement in OPP supplemented group reflected in a significant reduction in in serum TC, TAGs, LDL-C, VLDL-C levels and risk factor1 with concomitant increase of HDL-C as compared to BPA group(p<0.05).
9- There were no significant differences in serum iron and TIBC values in different experimental groups .
10- The results of this study reported that, Hb concentration and RBCs count showed a significant reduction in BPA group as compared to -ve control group. While supplementation with OPP significantly increases Hb concentration and RBCs count in G3, G4, G5 and G6 as compared to BPA group. On the other hand, TLC levels were significantly elevated in BPA group by 31.95% compared to -ve control group. While OPP dietary
supplementation significantly reduced TLC level in G3, G4, G5 and G6 by -7.28%, -12.20%, -14.07% and -16.63%,
respectively as compared to BPA group.
11- The histopathological examination of liver sections after administration of BPA (350 mg/kg b.w) twice weekly for 4 weeks illustrated pathological manifestations as bile duct massively hyperplasia with hypertrophy portal artery, dilated portal vein and scattered activated kupffer cells in blood sinusoid were obviously observed. Also, fatty vacuolation, atrophy of ductile epithelial lining and occluded portal vein by hyaline material which surrounded by inflammation were found. On the other hand, OPP supplementation showed marked improvement in all over the hepatocytes and restoring hepato-arcetucture pattern in a dose dependent manner.
12- The spleen microscopic examination of BPA group showed hemorrhaged bulges, decreased peri-arteriolar lymphoid sheath (PALS) numbers, size and cellularity and invaded blood capillary accompanied by fibrotic trabecula. On the other hand, OPP supplemented groups showed markedly improvement of splenic sinuses and cords pointed to significant improved in each of red and white pulps with rearranged splenic medullary tissue.