الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
Abstract
Water samples were collected seasonally from raw water and treated water at Kafr El-Shinawy and New Damietta drinking water treatment plants over the year 2018, to determine the different pollutants and their effects on water quality through chemical, physical, biological (phycological) analyses and detection of algal toxins.
The results of physico-chemical properties of water showed a noticeable difference in the trophic level of water, as the raw and treated water at New-Damietta WTP were significantly less polluted compared to raw and treated water at Kafr El-Shinawy WTP.
Raw water at New-Damietta WTP showed more species number compared to raw water at Kafr El-Shinawy WTP. Meanwhile raw water at Kafr El-Shinawy WTP had higher phytoplankton cell number.
Biochemical composition of phytoplankton showed a difference in chlorophyll a, lipids, proteins and carbohydrates concentration between phytoplankton at two water treatment plants.
The removal efficiency of New-Damietta WTP for water pollutants is higher than that at Kafr El-Shinawy WTP. The removal efficiency of TOC was very high at New-Damietta WTP compared with the negative values at Kafr El-Shinawy WTP. While THM compounds increased in treated water at both sites.
HPLC results showed that in raw water of Kafr El-Shinawy WTP the intracellular toxins are relatively higher than the extracellular toxins, whereas in treated water the extracellular toxins are higher.
Molecular studies confirmed that the most predominant phytoplankton species caused bloom at raw water at Kafr El-Shinawy WTP belongs to blue green algae. The microscopic and molecular genetic studies proved that this bloom belongs to Microcystis aeruginosa. The polymerase chain reaction (PCR) was to amplify the 16S rRNA and mcyA genes, the results of electrophoresis showed that all the isolates gave a clear band of 250 base pairs when using specialized primers of the 16S rRNA gene and a clear band 395 base pair size when using mcyA primers. Also, the cyanobacterial bloom appeared during winter belongs to Oscillatoria sp. and the electrophoresis results showed that this winter isolate gave a clear band of 1500 base pairs when using specialized primers of the 16S rRNA gene.