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العنوان
Evaluation of the Protective Effect of
Graviola on the Deleterous Effect of
Diabetes and Insulin Gene Expression in
Submandibular Salivary Glands of Albino
Rats :
المؤلف
Mokhtar, Sherihan Hamdy.
هيئة الاعداد
باحث / شريهان حمدى مختار
مشرف / سامية مصطفى كمال
مشرف / دينا صبرى عبد الفتاح
مناقش / نوال عبد الخالق شاهين
مناقش / ريهام مجدى محمد امين
الموضوع
Diabetal. Insulin. Gene Expression. Salivary Glands.
تاريخ النشر
2019.
عدد الصفحات
xi, 194, [5] P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
طب الأسنان
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة القاهرة - الفم والأسنان - Oral Histology
الفهرس
Only 14 pages are availabe for public view

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Abstract

Aim of the study: The present study was performed to investigate the protective effect of graviola against the deleterious effect of diabetes mellitus on the submandibular salivary gland of albino rats regarding the histopathological changes, oxidative stress, mucopolysaccharides, serum glucose level and insulin gene expression. Material and methods: Thirty adult male albino rats weighing about 150-250gm were used in this study and were divided in to 3 equal groups each of 10 animals. The rats of first group received a single IP injection of 0.1M sodium citrate buffer and served as control. The rats of second group were injected with streptozotocin 80 mg/kg body weight dissolved in 0.1 M sodium citrate buffer (pH 6.3) by single intraperitoneal injection. Diabetes was allowed to develop and stabilize in these STZ-treated rats over a period of 4-7 days. Diabetes was permitted to develop and become stable in group III animals by the same technique and then received a daily dose of 100mg//kg body weight of extracts of A. muricata every week in the animals. The animals were fed the normal laboratory diet and allowed drinking water ad libitum throughout the whole experimental period which lasted for 8 weeks. At the end of the experiment, the rats of the different groups were sacrificed and the submandibular salivary glands dissected out and processed for histological evaluation by Hematoxylin and Eosin, immunohistochemically for iNOS expression, histochemically by Alcian blue pH 2.5and PAS for carboxylated mucopolysaccharides and neutral glycoproteins. Also the specimens were examined for assessment of insulin gene expression using qRT-PCR. Histomorphometrical evaluation were carried out for acini number and surface area of both acini and ducts in histological sections. Quantitative assessment of histochemically and immunohistochemically stained sections were done in term of area pecent and optical density. Lastly, statistical analysis of summarized data was done by analysis of variance (ANOVA) and post hoc test. Results: histological examination showed typical normal histological picture of both parenchymal elements and connective tissue components in the submandibular salivary glands of control group. In group II (diabetic group), the submandibular salivary gland showed decrease in number of acini that displayed severe cytoplasmic vacuolaizations and decrease in cytoplasmic basophilia. The ducts showed hypertrophy of lining cells and their overall size .Basal striations were more conspicuous in striated ducts and persisted in excretory ducts. The vascularity of the salivary gland seemed to be increased with diabetes together with signs of interstitial edema.In graviola treated group, the acinar vacuolization was greatly diminished and the ducts regained mostly normal size and structure. However, few dysplastic features in the form of hyperchromatism, binucleation and increased N/C ratio were encountered. Moreover, the vasodilatation persisted and may became more exaggerated than the diabetic group. Immunohistochemically, examination of submandibular salivary gland of control rats revealed the predominant expression of iNOS in the duct system. Whereas, negative or minimal traces of the immunostain could be localized in the cytoplasm of the secretory terminal portion. In diabetic group, the submandibular salivary gland showed strong nuclear immunoreactivity in the acini that were negative in the control group and the cytoplasm showed obvious immunoreactivity of mild to moderate intensity. The ductal cells immunoreactivity involved all the nuclei and the cytoplasm together with the luminal salivary content. The immunoreactivity for iNOS was markedly diminished in acinar nuclei secondary to graviola treatment .However the blood vessels presented increased immunoexpression than the control and diabetic groups.Histochemically the acini of submandibular salivary glands of control group showed moderate to strong positive .