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العنوان
USE OF TISSUE CULTURE IN THE PRODUCTION OF SECONDARY METABOLITES from CHICORY (Cichorium intybus) PLANT /
المؤلف
SAYED,MARWA OTHMAN MOHAMED.
هيئة الاعداد
باحث / MARWA OTHMAN MOHAMED SAYED
مشرف / Laila Mohamed Helmi
مشرف / Abdelaziz Mohamed Hosni
تاريخ النشر
2020
عدد الصفحات
101p.:
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البساتين
تاريخ الإجازة
1/1/2020
مكان الإجازة
جامعة عين شمس - كلية الزراعة - نباتات الزينة والنباتات الطبية والعطرية
الفهرس
Only 14 pages are availabe for public view

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Abstract

The most relevant results of this research study may be summarized as follows:
• Clorox 10 % for 10 minutes was the best sterilant for chicory seed, before in vitro germination.
• Half-strength MS medium was the best germination medium for chicory seeds to grow strong seedlings (leaf length, width, and number/seedling) as an aseptic source for leaf explants used later on to induce callus.
• NAA at 3 mg/l plus IBA at 2 mg/l successfully produced desirable yellow friable callus from chicory leaf explant.
• NAA at 3 mg/l plus IBA at 2 mg/l under dark condition was the superior treatment for promotion of callus fresh and dry weights, moreover it gave the highest values for phenylalanine ammonia lyase (PAL) enzyme activity.
• NAA at 5 mg/l plus IBA at 2 mg/l gave the highest values for free amino acids, reducing sugars and polyphenol oxidase (PPO) enzyme activity, but it gave the lowest values for total soluble proteins.
• NAA at 2 mg/l was the superior treatment that gave the highest values for total phenolic and flavonoid compounds. Moreover, it gave the highest values for Guaiacol peroxidase (G-POD) enzyme activity.