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العنوان
The Association between Polymorphism in the ABCB1 Gene and Opioid Use Disorder in an Egyptian Sample \
المؤلف
Waheeb, Bishoy Mamdouh.
هيئة الاعداد
باحث / بشوي ممدوح وهيب ناشد
مشرف / نهلة السيد ناجي
مشرف / محمود ممدوح الحبيبي
مشرف / محمد حسام الدين عبد المنعم
تاريخ النشر
2019.
عدد الصفحات
179 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الطب النفسي والصحة العقلية
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - كلية الطب - طب المخ والأعصاب والطب النفسي
الفهرس
Only 14 pages are availabe for public view

from 179

from 179

Abstract

The current study is a comparative cross sectional case-control study. It was carried out at the substance use outpatient clinic of psychiatric institute of Ain shams university, Cairo. The sample was consisted of 2 groups; a case group, where 30 patients from both genders with age range of 18-65 years diagnosed as having opioid use disorder were included in the study. The second group was a control group which included 15 healthy volunteers matched for age, gender, socioeconomic class and present history of cigarette smoking.
All participants in case group were assessed using ICD-10 symptom checklist for mental disorders for clinical diagnosis of opioid use disorder, the English version was used, The Addiction Severity Index was used to assess the degree of severity of the opioid use disorder at the level of different parameters which include physical, occupational, addiction, legal, family, social and psychological problems.
DNA samples of all participants from both groups were obtained via buccal swabs and were stored in their original packs at -20ºc for DNA Extraction. Molecular genetic analysis of ABCB1 polymorphism was performed at the Oncology Diagnostic Unit-Medical Biochemistry Department- Ain Shams University. The DNA content of the samples was extracted by using Taqman probe SNP assay and Taqman master mix by Real time PCR technique. Details of the genotyping assay are described in subjects and methods chapter.
As regards the prevalence of ABCB1 gene polymorphism among the studied groups, we found that the commonest genotype among the addicts group was the Heterozygous C/T with a significant difference. Also we found a high statistical significant correlation regarding CC genotype between case and control groups. No significant difference was found between addicts and controls for the TT genotype.
Regarding the favorable and unfavorable alleles that predispose to opioid addiction, we found that the T allele is the favorable and protective allele for susceptibility of opioid addiction in contrast to the C allele.
While regarding the predictive value of ABCB1 gene rs1045642 polymorphism in opioid addiction as well as the type of addicted opioid substance, it was found that the presence of the ABCB1 gene rs1045642 polymorphism raises the probability to opioid addiction meaning that persons who possess this polymorphism are susceptible to opioid addiction than others, Also the presence of the studied polymorphism increases the probability to Tramadol addiction in contrast to Heroin addiction.
Regarding the correlation between different genotypes of the ABCB1 gene C3435T polymorphism among studied addicts group and different phenotypes of opioid addiction like the duration of addiction, maximum period of abstinence and the starting age of addiction, a significant data was not achieved.
Also regarding the correlation between different ABCB1 (C/T) gene polymorphism and different sociodemographic variables, there was no significant association other than gender as CC genotype was predominant in male gender followed by TT and the lowest percentage was for the CT genotype. Nearly equal numbers regarding CT and TT genotypes were found in the female gender. Therefore a high significant difference was achieved.
As regards the association between the ABCB1 gene polymorphism and the severity of the opioid use disorder, we found no significant association in the most of the investigated domains.