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العنوان
Incidence of Cytomegalovirus IgG antibodies and the role of Matrix Metalloprotinase-13 (MMP-13)-as a novel tumor marker- for Diagnosis of Breast Cancer in Egyptian Females /
المؤلف
Abd Elkader, Aliaa Mohamed Seif Elden.
هيئة الاعداد
باحث / علياء محمد سيف الدين عبد القادر
مشرف / / أحمد بركات بركات
مناقش / نيفين احمد عبد الحفيظ
مشرف / علي فهمي محمد السيد
تاريخ النشر
2019.
عدد الصفحات
89 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Molecular Biology
تاريخ الإجازة
22/1/2019
مكان الإجازة
جامعة عين شمس - كلية العلوم - ميكروبيولوجي
الفهرس
Only 14 pages are availabe for public view

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Abstract

Breast cancer is the fifth most common cause of cancer death (after lung cancer, stomach cancer, liver cancer, and colon cancer). Breast cancer is the most common invasive cancer in females worldwide. It accounts for 16% of all female cancers and 22.9% of invasive cancers in women. It was estimated that 18.2% of all cancer deaths worldwide, including both males and females, are from breast cancer.
MMP-13 known as Collagenase-3 is a member of collagenase subfamily initially identified in breast cancer. It was later found to be over-expressed in other malignant tumors, suggesting that MMP-13 is associated with aggressive tumors.
Cytomegalovirus (from the Greek cyto-, ”cell”, and megalo-, ”large”) is a genus of viruses in the order Herpesvirales, in the family Herpesviridae, in the subfamily Betaherpesvirinae. Human and monkeys serve as natural hosts. There are currently eight species in this genus including the type species human herpesvirus 5. Diseases associated with HHV-5 include glandular fever, and pneumonia. It is typically abbreviated as CMV. Viruses have been central to modern cancer research and provide profound insight into both infectious and non-infectious cancer causes and players in disease progression.
The aim of this study was to investigate the role of infection as a cause of carcinogenesis by estimating the incidence of anti-cytomegalovirus IgG antibodies in breast cancer patients. The study also aimed to evaluate the use of MMP-13 as a potential tumor marker in breast cancer.
All subjects enrolled in the study were recruited from Ain Shams University Specialized Hospital, and the laboratory work was conducted in Clinical Pathology Department, Immunology Unit of the Hospital.
The study included 50 breast cancer female patients (group I), 20 female patients with fibroadenoma of breast (group II) and 20 healthy age-matched female volunteers (group III). All individuals included were subjected to measurement of CA 15.3, Anti-CMV IgG and MMP-13.
A highly significant difference was detected between the three groups regarding MMP-13 and CA15.3. Statistical analysis revealed that group III (control group) had the highest MMP-13 while group II (fibroadenoma group) had the lowest MMP-13.
Regarding CA15.3, group I (breast cancer group) had the highest CA15.3 while group III (control group) had the lowest CA15.3.
Regarding CMV IgG status only one subject of the control group was negative for CMV IgG while all other subjects in the three studied groups were sero-positive for CMV IgG.
A significant statistical negative correlation was found between CA15.3 levels and MMP-13 levels in group I. No significant statistical correlation was found between CA15.3 levels and MMP-13 levels in either group II or group III.
Conclusively, the results of this study showed that MMP-13 is frequently present in the control group, while fibroadenoma group had the lowest MMP-13. CA15.3 is still the most useful serum tumor marker in patients with breast cancer. We found CMV in all subjects of the study denying a possible role in breast cancer association.
Recommendations and future study:
1. Future studies on a larger population sample size for use of MMP-13 as a diagnostics, prognostic, predictor of cancer outcome, target of therapy and monitoring treatment response of breast cancer is recommended to fully understand the function of MMP-13.
2. Conduction of other studies that depends on measuring MMP-13 by other specific techniques as PCR is preferable.
3. Inclusion of MMP-13 is advised in further studies, so more correlative results between MMP-13 expression and the tumor stage can be revealed.