الفهرس 
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Abstract
107 isolates of bacterial endophytes were collected from seven medicinal plants. Gram staining and microscopic examination revealed that eighty two isolates were Gram-positive (63 bacilli, 17 cocci and 2 filamentous) and 25 isolates were Gram-negative short rods. The
preliminary study for antibacterial activity against eight gram positive
and negative pathogenic bacteria (by cross streak method) indicated that
bacterial endophytes of three plants (Ocimum basilicum, Rosmarinus
officinale and Withania somnifera) had excellent antagonistic properties
with the tested microbes. In secondary screening, endophytic bacteria
isolated from Ocimum basilicum, Rosmarinus officinalis and Withania
somnifera showed considerable antimicrobial activity. The most
bioactive endophytic bacteria and their harboring medicinal plants were
selected for further investigations. Based on the results obtained, and
correlating with the results from morphological and biochemical tests,
the endophytic bacteria isolated from Ocimum basilicum, Rosmarinus
officinalis and Withania somnifera were Bacillus subtilis US1, Bacillus
subtilis US2 and Klebsiella variicola US respectively. The 16S rRNA
gene sequences of endophytic bacteria reported in this study have been
deposited in the GenBank database under accession numbers:
MH092890 (Bacillus subtilis US1), MH093646 (Bacillus subtilis U