الفهرس | Only 14 pages are availabe for public view |
Abstract This studying is refering to identified of an easy and eco-friendly way for biosynthesis of nanoparticles, nanocomposite. Using isolates to metal ions leads to nanoparticles formation and using sonic vibro cell for synthesis nanocomposite. Nanoparticles were in the range at about 5-130 nm dimension. Nanoparticles and nanocomposite were examined utilizing Ultra Vilot-Visible spectroscopy, Transmission Electron Microscopy “TEM”, X-ray Diffraction “XRD”, and Fourier Transform Infrared Spectroscopy “FTIR” analysis, protein fractions and Maldi-TOF Analysis to identify their effectiveness in antimicrobial activities. For production of nanoparticles and nanocopmosite different human pathogenic bacterial strains were used. All the microbes were inhibited at low concentrations of the tested nanoparticles. The inhibition caused by nanoparticles was gradually increased with E. coli and Pseudomonas aeruginosa (Gram negative) compared with Staph. aureus and MRSA (Gram positive). The antitumor activity of HCT-116 (colon carcinoma cell) cancer cells. Where the results indicate high cytotoxic effect for synthesized silver nanoparticles 500μl- Zinc oxide nanoparticles 500 μl with the half maximal inhibitory concentration IC50=73.7 μl, nanocomposite against cancer HCT-116 colon carcinoma cell line. For water purification from heavy metals and also antibacterial, the results indicate that the maximum adsorption of heavy metals with almost all adsorbents were obtained with an adsorbent dosage was considered the optimum time at about 15 minute. Nanocomposites showed higher growth inhibitory effect on Shigella, compared to Salmonella. Salmonella and Shigella growth was inhibited by 100 % inhibation when exposed to 2.17 mg/mL of silver, zinc oxide and titanium dioxide nanoparticles. |