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العنوان
Improvement of the Antimicrobial Properties of
Nanoparticles and Application in Water Purification /
المؤلف
El-Kahky,Dina Mohammed Sherif Moheb.
هيئة الاعداد
باحث / Dina Mohammed Sherif Moheb El-Kahky
مشرف / Saadia Mohamed Easa
مشرف / Magdy Attia Mohamed
مشرف / Nemat Mostafa Awad
تاريخ النشر
2019
عدد الصفحات
314p.:
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الكيمياء الحيوية ، علم الوراثة والبيولوجيا الجزيئية
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - كلية العلوم - الميكروبيولوجى
الفهرس
Only 14 pages are availabe for public view

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from 314

Abstract

This studying is refering to identified of an easy and eco-friendly way for
biosynthesis of nanoparticles, nanocomposite. Using isolates to metal ions
leads to nanoparticles formation and using sonic vibro cell for synthesis
nanocomposite. Nanoparticles were in the range at about 5-130 nm
dimension. Nanoparticles and nanocomposite were examined utilizing
Ultra Vilot-Visible spectroscopy, Transmission Electron Microscopy
“TEM”, X-ray Diffraction “XRD”, and Fourier Transform Infrared
Spectroscopy “FTIR” analysis, protein fractions and Maldi-TOF Analysis
to identify their effectiveness in antimicrobial activities. For production of
nanoparticles and nanocopmosite different human pathogenic bacterial
strains were used. All the microbes were inhibited at low concentrations
of the tested nanoparticles. The inhibition caused by nanoparticles was
gradually increased with E. coli and Pseudomonas aeruginosa (Gram
negative) compared with Staph. aureus and MRSA (Gram positive). The
antitumor activity of HCT-116 (colon carcinoma cell) cancer cells. Where
the results indicate high cytotoxic effect for synthesized silver
nanoparticles 500μl- Zinc oxide nanoparticles 500 μl with the half
maximal inhibitory concentration IC50=73.7 μl, nanocomposite against
cancer HCT-116 colon carcinoma cell line. For water purification from
heavy metals and also antibacterial, the results indicate that the maximum
adsorption of heavy metals with almost all adsorbents were obtained with
an adsorbent dosage was considered the optimum time at about 15
minute. Nanocomposites showed higher growth inhibitory effect on
Shigella, compared to Salmonella. Salmonella and Shigella growth was
inhibited by 100 % inhibation when exposed to 2.17 mg/mL of silver,
zinc oxide and titanium dioxide nanoparticles.