الفهرس 
IntroductionOnly 14 pages are availabe for public view
Abstract
SUMMARY
emale pattern hair loss (FPHL) is the most common hair
loss disorder in women. The importance of this disease
comes from the intense emotional distress and disrupted selfimage
caused by the hair loss where there is progressive
nonscarring replacement of terminal hair follicles over the
frontal and vertex regions by miniaturized follicles, which leads
progressively to a visible reduction in hair density.
Treatment of FPHL remains a challenge as no treatment
is either completely curative or preventive. Platelet-rich plasma
(PRP) provides an option for patients recalcitrant to the
currently approved therapies; minoxidil and finasteride.
Moreover, with the annoying daily applications required for the
proper use of minoxidil and the growing concern over potential
finasteride-induced side-effects, an additional treatment would
be advantageous.
After centrifugation of the whole blood, platelets can be
isolated; growth factors can be concentrated and injected into
the hair follicle and surrounding area. Evidence has suggested
that these concentrated growth factors can promote
angiogenesis and vascularization, increase the duration of the
hair growth phase and accelerate hair regrowth.
As there are many methods for the preparation of PRP,
the most suitable for AGA therapy is not yet clear. To isolate
F
Summary
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PRP, some laboratories use a single-spin method with success,
however many use a double-spin method. Also, there is a lack
of consensus regarding the mode of preparation, addition of
activators, centrifugation time and speed, platelet concentration
attained, and volume of blood and PRP used.
Our study aimed to compare the efficacy between single
and double spin PRP injections in treatment of female pattern
hair loss and to assess the relationship between the
concentrations of vascular endothelial growth factor (VEGF) in
the PRP preparation and the treatment results obtained.
This study was done on 15 female patients with FPHL.
All were subjected to detailed history taking and examination to
exclude any patients with signs of hyperandrogenism. Each
patient was subjected to intradermal injection of PRP (prepared
by double-spin method) into the right half of the scalp, in
addition to intradermal injection of PRP (prepared by singlespin
method) into the left half of the scalp in the form of three
treatment sessions three weeks apart. Furthermore, VEGF
concentration was measured using ELISA kit in both the singlespin
and double-spin prepared PRP before and after activation
with calcium gluconate in the first treatment session and only
after activation in the third treatment session. Evaluation of
treatment response was done through comparing patients’
global photographs, assessment of patients’ satisfaction and
folliscopic assessment before and after treatment.
Summary
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The obtained data were tabulated and statistically
analyzed. Our results showed clinically significant
improvement by comparing patients’ photographs in most of the
treated patients and high mean patients’ satisfaction with low
PRP’s adverse effects profile. In addition, folliscopy revealed
increased median terminal hair density in both the right and left
halves of the scalp following treatment sessions. However,
there was only statistically significant change of the terminal
hair density in the right half of the scalp following treatment
sessions. This suggests that the double-spin prepared PRP
could yield better results in the treatment of FPHL than the
single-spin method. VEGF concentration did not differ
significantly between double-spin and single-spin prepared
PRP, also it did not differ significantly upon calcium activation.
In conclusion, PRP is an effective treatment modality for
FPHL. Double-spin prepared PRP could yield better results in
the treatment of FPHL than the single-spin method. Yet, further
studies discussing different PRP preparation protocols and
involving a larger number of cases are needed to minimize
statistical errors and to detect the most accurate PRP
preparation method with the greatest yield of growth factors.