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العنوان
COMPARISON BETWEEN DIFFERENT TISSUES USING
INNOVATED METHODS IN EXTRACTION OF DNA FOR
IDENTIFICATION OF CORPSE IN CASE OF
ENVIRONMENTAL DISASTERS EXPLOSIONS AND FIRES /
المؤلف
Naser,Jihan Ahmed Abd Elwahab El Sayed.
هيئة الاعداد
باحث / Jihan Ahmed Abd Elwahab El Sayed Naser
مشرف / Mostafa Hassan Ragab
مشرف / Sherien Salah Ghaleb
تاريخ النشر
2019
عدد الصفحات
221p.:
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
العلوم البيئية (متفرقات)
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - معهد البيئة - العلوم الطبية البيئية
الفهرس
Only 14 pages are availabe for public view

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from 221

Abstract

Every disaster is unique and involves interplay of different factors
and circumstances such as nature of disaster, number of victims and
extent of body fragmentation that eventually challenges the disaster
response planning. These events need special planning and preparation
designed specifically for situations with potentially thousands of
deceased victims.
Developments in molecular genetics have allowed studying the
person-to-person differences in parts of DNA that are not involved in
coding for proteins, and it is primarily these differences that are used in
forensic applications of DNA typing for personal identification. Sample
preference for DNA analysis can help in proper management of the
disaster. Since a disaster is a chaotic environment that can complicate
definite identification of the remains. With some planning, and proper
sample selection we can reduce stress for those involved in the
identification procedure thus increasing the probability that all recovered
samples are identified.
The goal of this work is to decide which human tissue is preferred
over the other for DNA tests for human identification as regards the
available samples recovered from different disasters including
environmental disasters, fires and explosions. Thus we can ensure precise
and quick identification of victims saving time resources and reduce
stress of relatives by delivering results accurately and quickly as possible.
Furthermore reducing the hazards of failure in victim identification.
This cross sectional laboratory based study was conducted at the
Egyptian Forensic Medicine Authority (EFMA) at Sayda Zaynab. From
May 2016 to April 2017 on 246 samples and tested using manual Qiagen extraction kit, Prep Filer extraction kit or Qiagen automated kit using
EZ1. Extracted DNA was then quantified using Quatifiler® Human DNA
Quantification kit by Step one Real Time PCR.
Samples were amplified using (AmpFlSTR ® Identifiler ® Plus)
PCR Amplification Kit (Life Technologies Applied Biosystems) and
AmpFlSTR ®Y Filer Amplification Kit (Life Technologies Applied
Biosystems). Amplified PCR products were run electrophorethically on a
3130xl or 3500 Genetic Analyzer (Life Technologies Applied
Biosystems) and finally analyzed.