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العنوان
Evaluation of Ginger and Monocationic Bithiophene Activity against Dimethylhydrazine-Induced Colon Cancer in Male Albino Rats /
المؤلف
Mohammed,Mohammed Ahmed.
هيئة الاعداد
باحث / Mohammed Ahmed Mohammed
مشرف / Nadia Mohamed El-Beih
مشرف / Wael Mohamed El-Sayed
مشرف / Shaymaa Mohmoud Yahya
تاريخ النشر
2019
عدد الصفحات
205p.;
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علم الحيوان والطب البيطري
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - كلية العلوم - علم الحيوان
الفهرس
Only 14 pages are availabe for public view

from 205

from 205

Abstract

The present study aimed to evaluate the antioxidant and antiproliferative effect of ginger extract (GE) and a novel bithiophene (BT) against HCT-116 colon cell line and understand the molecular mechanism(s) through which BT and GE exert their antitumor activity. The results showed that GE had a good antioxidant capacity and higher DPPH˙ and hydroxyl radical scavenging abilities than the novel BT. BT was the most cytotoxic agent against HCT-116 cancer cell. GE and BT act as anticancer agent through upregulation of FasL, TRAIL, p53 and caspase-8 and downregulation of Bcl-2 and survivin genes. Rats were randomly divided into (8) groups. group 1 untreated control. group 2 left untreated for 9 weeks then treated with cisplatin (CP) (5 equal doses of 2.5 mg/kg, i.p.). group 3 left untreated for 9 weeks then treated with GE (300 mg/kg, p.o., 3 doses/week). group 4 left untreated for 9 weeks then treated with
BT (2.5 mg/kg, i.p., 3 doses/week). group 5 was s.c injected with
dimethylhydrazine (DMH) (20 mg/kg) for 9 weeks. Groups 6,7,
and 8 administered DMH for 9 weeks then treated with CP, GE,
or BT, respectively, for the remaining 21 weeks. Treatment of rats
with DMH resulted in 100% tumor incidence and 2.3 tumor/rat. A
significant elevation in serum ALT, urea and creatinine and a
decreased in body weight gain. A significant decrease in hepatic
GSH level, CAT, SOD, GST, GR activities and GSH content and
γ-GT activity in kidney. Histopathological examination of colon
of DMH-treated rats revealed adenomatous polyps with
inflammatory reaction, polymorphism, clear mitosis,
inflammatory cells infiltrating the mucosa and submucosa. Both
muscularis and serosa were free from inflammatory cells but
involved in cancer formation. Anaplastic changes and cystic
formation together with few hemorrhage and inflammatory
reaction infiltrating muscularis. GE and BT were able to mitigate
all the previous injurious effects of DMH. The alleviation offered
by GE and BT was better than that shown after CP administration.
Thus, this study proved that GE and BT ameliorated the DMH
induced-colon tumors and were hepatoprotective and
renoprotective agents.