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The aim of the present work is the development and introduction of modern analytical techniques with high sensitivity and selectivity with low cost for diagnosis for liver cirrhosis by assessment of 3-Nitrotyrosine in serum and assessment of amirolide HCl in serum and urine samples.
The study is divided into 3 chapters:
Includes a general introduction on lanthanides and other methods used for assessment of 3-Nitrotyrosine in serum and assessment of amirolide HCl in serum and urine samples.
Describes highly selectivity and sensitivity spectrofluorimetric method for assessment of 3-Nitrotyrosine as a biomarker for early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) disease based on quenching the luminescence intensity of the optical sensor (Eu(TTA)3Phen) after addition of different concentrations of 3-Nitrotyrosine in DMSO at pH=9 and λex=375nm, making linear relation between fluorescence intensity against inverted concentration and making wide linear working range (8.8 x 10-6 - 3.4 x 10-9 ) mol L-1 and the limit of detection (LOD) and quantitation limit of detection (LOQ) are (1.6 x 10-9 and 4.9 x 10-9 mol L-1).
The rapid and sensitive spectrofluorimetric method is used for the determination of Amiloroide hydrochloride in different persons in serum sample. The method depends on the quenching of [Pt (pyc)2] complex photo probe by amiloroide hydrochloride concentration in water. The linear range is 1.6×10-9– 2.1×10-6 mol L-1, and the limit of detection (LOD) and quantitation limit of detection (LOQ) are (7.50x 10-10 and 2.27x 10-9 mol L-1.