الفهرس 
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Abstract
Canine parvovirus (CPV) is the most significant viral cause of acute hemorrhagic enteritis in puppies.
The present work aims to answer some questions about canine parvovirus through, Investigation clinical signs and determination of the percentage of infection; isolation and identification of CPV and identification of some risk factors related to CPV infection from target dogs. Evaluation of the efficacy of the local and imported CP vaccines in addition to investigate the efficiency of PIND-ORF (zylexis) in vaccinated and experimentally infected dogs.
The obtained results showed that:
1- Enteric form was the most common presenting clinical signs including anorexia, fever, vomiting, bloody diarrhea, and lethargy. These findings were described in 120 out of 170 examined dogs representing a percentage of 70.58%.
2- Post mortem examination in three died puppies affected with canine parvovirus infection showed diffuse hemorrhagic enteritis, thickened intestinal wall and segmentally discolored with denudation of intestinal mucosa and watery material within the stomach and intestinal lumen.
3- Rapid detection of CPV antigen by immunochromatographic (IC) test was performed on fecal swabs obtained from all dogs revealed that 69 out of 170 samples (40.6%) had CPV antigen.
4- Viral isolation on tissue culture revealed that
a. Forty-eight out of 120 fecal samples from clinically suspected infected animals were positive and had progressed cytopathic effect (CPE) [cell rounding and aggregation followed by cell lysis and detachment from the culture surface]. CPE was started by the 5th day post infection of Vero cell culture and completed within the 7th to 8th day later.
b. Identification of virus by Rapid slid agglutination test (RAT), Virus neutralization test (VNT) and direct fluorescent antibody technique (FAT) were confirmed the presence of CPV.
5- Detection of CPV2 by conventional PCR applied on three samples positive by IC only and three positive samples by both of IC and TC in addition to 6 negative samples revealed that all positive samples by IC and or TC were positive by PCR (630 bp for CPV and 427 bp for CPV 2a).