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العنوان
Development of Composite Nanofibers - Based Drug Delivery Systems for Wound Treatment /
المؤلف
Mansour, Sarah Mansour Gaber.
هيئة الاعداد
باحث / ساره منصور جابر
مشرف / اسامه يوسف عبدالله
مشرف / ماجده عبد السميع المسيك
مشرف / مي سعد فريج
الموضوع
Pharmaceutics. Wound Treatment. Drug Delivery.
تاريخ النشر
2019.
عدد الصفحات
136 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
العلوم الصيدلية
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة الاسكندريه - كلية الصيدلة - صيدلنيات
الفهرس
Only 14 pages are availabe for public view

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from 148

Abstract

Skin is the largest organ of the human body. It performs several functions such as; defensing the body against the entry of chemicals and microorganisms, providing a barrier to the loss of fluids and salts as well as helping in regulation of body temperature. The large surface area of the skin makes it a potentially interesting route for drug delivery.Millions around the world suffer from acute and chronic wounds. Treatment become a major health issue. The main objective in the management and treatment of wounds is to achieve quick healing of the skin.Wound dressings represent a very important segment in the medical and pharmaceutical wound care system. Their role is to keep the wound dry by allowing evaporation of wound exudates and preventing entry of harmful bacteria into the wound surface. It has now been shown, that having a warm moist wound environment, effective oxygen circulation around the wound achieve more rapid and successful wound healing process.Oneof the principal research drivers in the field of wound care development focuses on the manufacture of wound dressings in the form of nanofibrous meshes. Nanofibrous meshes offer a good starting point toward the development of a synthetic scaffold able toreproduce the structure of the natural ECM. In fact, due to their nanometer diameter and random alignment within the mesh, fibers tend to imitate the fibrous architecture of the natural ECM. In addition, nanofibrous meshes have been shown to promote the hemostasis of injured tissues thanks to the presence of small interstices and the high surface area of the fibers. The high-surface area is also essential for fluid absorption, enhanced dermal drug and antimicrobial delivery and it provides the opportunity to modify the surface of the fibers with specific chemical functionalities. Nanofibrous meshes show high interconnected porosity (60–90%), allowing cell respiration and high-gas permeation and prevention of wound desiccation and dehydration.Various techniques, including phase separation or self-assembly, can be used for the fabrication of the meshes, but electrospinning is most frequently chosen because it is a simple, cost-effective, and versatile process. Based on the application of a high voltage to theselected polymer solution or melt, nanometric filament formation is induced as the polymer is drawn towarda collector. After a certain time, a nanofibrous structure made of polymeric non-woven or aligned fibers can be collected.Ideally,the meshes shouldbe able to actively initiate the healing processes, while reducing the bacterial contamination and treating infection only if necessary. Since the dressing is designed to be removed once the wound has healed, the mesh should promote cell migration and proliferation within the wound bed while preventing tissue ingrowth within the fibrous structure.Considerable attention has been focused on the development of composite nanofibers-based drug delivery systems for wound treatment as it was shown that nanofiber wound dressing materials displayed advanced properties that can enhance wound repair in comparison with other dressings. VerapamilHClhas been chosen as a model drug for the current study. VerapamilHClis characterized by high water solubility, freely soluble in organic solvents. VerapamilHClconsiderably increasesrate of wound closure.It is an excellent scar modulator that prohibits the development of hypertrophic scars and keloids in the postoperative period. It exhibits great potential for controlling the extracellular matrix biosynthesis so its topical administration will be promising .On the other hand ,it is a challenging drug to be incorporated
117in the form of nanofibrous matsas it is the first instance of encapsulating verapamil HClinto nanofibrous meshesfor wound healing . So,the current work aimed to develop and characterize two differentdrug delivery systems ofverapamilHClin the form ofnanofibrous mats bothfrom natural origin one from brown algae using SA, another one fromCorn or maize (Zea mays L.) : zein obtained by electrospinning technique.Evaluation of different placebo formulations with different concentrations to achieve the best obtained nanofibrous meshes for drug loading in both systems was assessed, In addition, loading verapamilHClin the optimum chosen placebo mats by electrospinning of verapamil HClin polymeric solutionswas also evaluated.Furthermore, the effect of polymer concentration and drug concentration on thefiber morphology and diameter was also studied. Comparative invitro drug release profiles of two different verapamil HClincorporatingnanofibrous matswere assessed. In addition,comparative in-vivo evaluation of the formulated verapamilHClloaded nanofibrous mats in the treatment of skin woundsin rats was assessed in both nanofibrous meshes.