الفهرس 
IntroductionOnly 14 pages are availabe for public view
Abstract
Modern analytical techniques for early diagnosis of some chronic diseases
The study presented in this thesis include development and the combination between some techniques and technologies such as Analytical chemistry, Biochemistry, Photochemistry and Nanotechnology for the determination of some active material (e.g. Folic acid, Progesterone, Testosterone, Vitamin D3, Warfarin the carbohydrate antigen CA 15-3 and the prostate-specific antigen PSA as biomarkers for the determination of some chronic diseases using some real samples.
The thesis is divided into 6 chapters:
Chapter One:
Includes a general introduction on lanthanides, optical sensors, fluorescence , chronic diseases and other methods used for determination of Folic acid, Vitamin D3, Progesterone, Testosterone, the carbohydrate antigen CA 15-3 and the prostate-specific antigen PSA as biomarkers for some chronic diseases using some real samples.
Chapter Two:
Describes a new nano-optical sensor Tb3+-3-Acetylindol complex for assessment of Folic acid. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated. Under optimal conditions, the luminescence intensity of Tb3+-3-Acetylindol complex is inversely proportional to the concentration of Folic acid in range (1.28×10-6 – 2.49 × 10-9) mol L−1, ( r = 0.999 ). The detection limit (S:N=3) ( LOD) and quantitation limit of detection (LOQ) are (4.0 ×10−9and 1.21×10-8) mol L-1 respectively. This method is used for early diagnosis of some chronic diseases.
Chapter Three:
Describes a new nano-optical sensor Tb3+-3-Acetylindol complex for assessment of Progesterone, Testosterone and Vitamin D3. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated. Under optimal conditions, the luminescence intensity of Tb3+-3-Acetylindol complex is inversely proportional to the concentration of Progesterone, Testosterone and Vitamin D3 in ranges (5 x 10-9-1.9 x 10-6 , 5 x 10-9-2.8 x 10-6 , 5 x 10-9-4.2 x 10-6) mol L−1, ( r = 0.999 ). The detection limit (S: N=3) ( LOD) and quantitation limit of detection (LOQ) are (1.5 x 10-9 , 3.0 x 10-9, 1.6 x 10-9) mol L-1 and (4.5 x 10-9, 9.0 x 10-9, 2.8 x 10-9 ) mol L−1 respectively. This method is used for early diagnosis of some chronic diseases.
Chapter Four:
Describes a new nano-optical sensor Eu-Doxycycline HCl complex for assessment of the prostate-specific antigen PSA as biomarker for prostate cancer using some real samples. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated. Under optimal conditions, the luminescence intensity of Eu-Doxycycline HCl complex is inversely proportional to the concentration of the prostate-specific antigen PSA in range 1.15X 10¬-6 – 3.08 x 10-9 molL−1, ( r = 0.999 ). The detection limit (S:N=3) ( LOD) and quantitation limit of detection (LOQ) are 2.17 x 10-6 molL-1 and 6.62 x 10-6 molL-1 respectively. This method is used for early diagnosis of some chronic diseases.
Chapter Five:
Describes a new nano-optical sensor Eu-Oxytetracycline HCl complex for assessment of the carbohydrate antigen CA15-3 as biomarkers for the breast cancer using some real samples. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated. Under optimal conditions, the luminescence intensity of Eu-Oxytetracycline HCl complex is inversely proportional to the concentration of the carbohydrate antigen CA 15-3 in range 0.11-50.29 UL−1, (r = 0.999). The detection limit (S:N=3) ( LOD) and quantitation limit of detection (LOQ) are 0.109 U L-1 and 0.27 UL-1 respectively. This method is used for early diagnosis of some chronic diseases.
Chapter Six:
Describes novel, simple, sensitive and selective spectrofluorimetric method for the determination of warfarin sodium in pharmaceutical tablets, urine and serum samples using optical sensor Eu3+-ACAC. The warfarin sodium can remarkably quench the luminescence intensity of Eu3+-ACAC complex in DMF at pH (6.43) and λex= 395 nm. The quenching of luminescence intensity peak of Eu3+-ACAC complex at λem = 616 nm is proportional to the concentration of warfarin sodium a result that suggested profitable application as a simple optical sensor for warfarin sodium assessment. The dynamic ranges found for the determination of warfarin sodium concentration are (1.6×10-9– 2.1×10-6) mol L-1. The limit of detection (LOD) and quantitation limit of detection (LOQ) are (7.50x10-10 and 2.27x10-9) mol L-1 respectively