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العنوان
Study The Potential Role Of Some
Nutraceuticals in The Management Of
Schizophrenia in The Experimental Rat Model /
المؤلف
Mostafa, Mona Elsayed Ahmed.
هيئة الاعداد
باحث / مني السيد أحمد مصطفي
مشرف / سميحة محمد عبد الدايم
مشرف / هناء حمدى أحمد
مشرف / أسماء مجدي زعزع
تاريخ النشر
2019.
عدد الصفحات
250p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الحيوان والطب البيطري
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - كلية البنات - قسم علم الحيوان
الفهرس
Only 14 pages are availabe for public view

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Abstract

Summary and conclusion
Schizophrenia is a heterogeneous psychiatric disorder that affects an average of approximately 1% of the World’s population making it the seventh most costly medical illness in the globe. It is a polygenetic disorder that is associated with environmental and neuro-developmental vulnerability factors, making it a highly complex ailment characterized by positive, negative and disorganized symptoms.
The symptoms shown by schizophrenic patients that are regarded as positive include auditory and visual hallucinations, delusions, conceptual disorganization, thought disorder and paranoia. The negative symptoms include emotional blunting, anhedonia, avolition, poverty of thought and content of speech, as well as depression. The cognitive dysfunctions associated with this debilitating psychiatric disorder include impaired executive function, working memory and attention.
The present study was contructed to elucidate the antipsychotic efficacy of some nutraceuticles (CoenzymeQ10 (CoQ10) and Pumpakin seed oil (PSO)), in the ketaminized rats as a good experimental model for schizophrenia. A total number of 60 male albino rats weighting 120-150g (3 months old) were used in the current study. The animals were classified into six experimental groups (10 rats/ group).
-The first group: served as negative control group.
-The second group: served as corn oil group
-The third group was administered orally with ketamine in a dose of 25 mg/kg b.wt./day for 120 days and served as positive control group (ketaminized group).
-The fourth group was administered orally with ketamine for 60 days and then treated orally with CoQ10 200 mg/kg b.wt./day simultaneously with ketamine for other 60 days. This group served as ketaminized group treated with CoQ10.
-The fifth group was administered orally with ketamine for 60 days and then treated orally with pumpakin seed oil(PSO) 40 ml/kg b.wt./day simultaneously with ketamine for other 60 days. This group served as ketaminized group treated with pumpakin seed oil (PSO).
-The sixth group was administered orally with ketamine for 60 days and then treated orally with respridone 6 mg/kg b.wt. /day simultaneously with ketamine for other 60 days. This group served as ketaminized group treated with respridone.
The therapeutic potential for the suggested treatments was evaluated in the experimental animal model for schizophrenia by behavioral tests, biochemical and hisopathological investigation of brain tissue:
1) Behavior tests included (FST) and Rota-Rod test before and after treatment.
2)Brain biochemical markers included serotonin (5HT)¸dopamine (DA)¸epinephrine(EP)¸ glutamate receptor (GR1) and glutamic acid(GA) contents. Also the brain contents of the neurotrophic factor (BDNF)¸ antiapoptic marker (Bcl-2) and apoptic marker (Caspase-3) were estimated by ELISA.
3)Histological examination of brain tissue section stained with hematoxylin and eosin stains was also done.
The results of the present study were as follows:
1-The ketaminized group revealed significant increase in the immobility time in FST and significant decrease in mobility time in Rota-Rod test.
2- Significant increase in the brain content of serotonin ¸dopamine, epinephrine and glutamate receptor while significant decrease in the brain content of glutamic acid in the ketaminized group were recorded.
3-Significant reduction in the brain content of BDNF , Bcl-2 and significant increase in Caspase-3 were detected in the ketaminized group.
4-Histological investigation of the brain tissue section of the ketaminized rat showed neuronal damage and congestion in the blood vessels and capillaries of the cerebral cortex, striatum and cerebellum associated with neuronal degeneration in the striatum.
5- ketaminized group treated with the Coenzyme Q10, pumpakin seed oil or respridone revealed significant improvement in the animal’s behavior and in the biochemical markers in addition to the obvious restoration of the histological feature of the brain tissue. These findings were documented by the significant decrease in the immobility time in the FST, significant increase in the mobility time in the Rota-Rod test , significant decrease in the brain content of serotonin, dopamine, epinephrine, glutamate receptor and Caspase-3. In addition, the significant increase in the brain content of glutamic acid , BDNF and Bcl-2 were detected in the treated groups.
Histological description of the brain tissue appreciated the biochemical results as indicated by the marked improvement in the brain histoarchitecture in the treated groups.