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العنوان
THE USE OF SOME AGRICULTURAL RESIDUES IN SURFACE CULTIVATION OF BARLEY PLANTS BY MICROORGANISMS /
المؤلف
SOUD, NOURA SOUDY HASSAN.
هيئة الاعداد
باحث / NOURA SOUDY HASSAN SOUD
مشرف / EL-SHAHATT MOHAMMED RAMADAN
مشرف / MAGDY MOHAMMED SHAFIK
مناقش / MOHAMMED ZAKARIA SEDIK
تاريخ النشر
2019.
عدد الصفحات
97p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - كلية الزراعة - الميكروبيولوجيا الزراعية
الفهرس
Only 14 pages are availabe for public view

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Abstract

A number of barley grains in 100 g were ranged between 2550 to 2620 grains. This test was carried out in order to know the number of grains in each tray.
The germination percent of 25 grains of barley and repeated 6 times was recorded after 24 hours of incubation at 25 ºC. The germination percentages reached 88%. This mean that approximately all grain of barley can germinate during growth period.
The total bacterial and fungal counts were recorded in the agricultural residues (rice straw and bagasse) before used. Results obtained revealed that the microbial counts in rice straw were less than estimated in bagasse. Where, the counts reached to 2.6* 104 CFU g-1 rice straw and the fungal counts to 1.4* 102 CFU g-1 rice straws. While in bagasse the counts were high and reached to 5.8*106 CFU g-1 bagasse. The fungal counts reached to 3.0*104cfu g-1 bagasse. The data indicate that, the microbial counts found in barley grains reached to 5.3*104 CFU g-1 grain and the fungal counts reached to 1.6*103 CFU g-1 grains.
The microbial percent was approximating similar in both residuals except, spore-forming bacteria and yeast. Where, the spore former percent was higher in rice straw (56%) than in bagasse (43%). While, the yeast percent was lower in rice straw (6%) than in bagasse (18%).
The microbial count recorded in HBS during growth indicate that, at the beginning, the counts were depending on the type and length of agricultural residual used as well as the type of inoculation (non, single strain or mixed strain).
Data recorded revealed that in rice straw, show the highest counts (2.8*104 CFU g-1) were estimated in fine size and the count decrease by increasing the residual length. The decrease reached to -57% and – 85.7% in control medium and rough length.
Data revealed that mixing an inoculation of Ps. fluorescens with S. cerevisiae increased the bacterial counts by 189.8% and the fungal counts decreased by -26% at the end of sprouted barley.
The counts estimated with bagasse residual as control treatment (without inoculation) were high at zero time (3.5*105 CFU g-1) and increased during the growth period reached to 9.8*105 CFU g-1at the end of HBS growth period .While, with inoculation treatments (single or mixed) the bacterial counts were more high and reached to 5.0, 6.6 and 8.2* 105 CFU g-1 at zero time with Ps. fluorescens, S. cerevisiae and Ps. Fluorescens + S. cerevisiae respectively. Moreover, the counts increased by increasing the HBS growth period by 91.8%, 119.0% and 172.2% depending on the same treatment applied as above mention.
The fungi counts were low from the beginning and were decreased during HBS growth period.
The vegetative qualities of hydroponic barley sprout (HBS) show no significant difference in all vegetative characters of HBS after 8 days of germination on rice straw or bagasse residual.
The fresh weight of HBS grown on rice straw data revealed that the best size of the agricultural residual is a fine size (≥1.0 cm2), and the best treatment was composite inoculum (Ps. fluorescens + S. cerevisiae). While the lowest fresh weight obtained was recorded with large length “Rough straw” (≥ 2.5*5.0 cm2) especially with inoculation treatment single strain or mixed strain (Table 6).
In addition, the highest root length recorded in both of rice straw or bagasse reached 5.5 and 6.8 cm after 8 days of HBS .
The data obtained revealed that the optimum length for HBS was a fine size (≥1 cm) for Ash, Lipid, Fiber and protein contents. While, in case of microbial inoculate, Ps. fluorescens inoculation was more suitable of rice straw, where, its increased ash content by (5.3 %), total lipid (8.0%), and fiber content (31%) and protein content (4.5%), respectively.
The values of nitrogen-free extracts (NFE) were depending upon the texture of residual tested and type of microbial inoculation. In rice straw texture, the low NFE value was recorded in fine size (> 1.0 cm). But, in inoculum treatment, the NFE was low with Ps. fluorescens. (- 19%), S. cerevisiae (1.40%) and Ps. fluorescens + S. cerevisiae (12.5%), respectively.
The DM, in non-inoculated HBS, decreased through the growth period (8 days). The percent decrease was increased according to the rice straw size.
The results of total carbohydrate and soluble carbohydrate indicated that, the total carbohydrate content did not change between rice straw sizes. But, in cases of Pseudomonas spp. and Saccharomyces spp. inoculum, the content increased but in low percentage. In barley sprouting, starch in catabolized to soluble sugars for supporting the metabolism and energy requirement of the growing plants for respiration and cell wall synthesis.
In bagasse residual, the values of ash, lipid, fiber, protein contents in un-inoculated ones were decreased depending on the length of bagasse. The decreased percentages reached 17% with Ash content, 43% with lipid content, 29.4% with fiber content and 23% with protein content respectively. Similarly the decrease was recorded in inoculation treatments in spite of type of inoculation.
The percent increase in NFE reached 21.0 % and 32.0% when HBs germinated on medium and rough length, respectively.
The quantity of fresh HBS obtained per 500 g barley grain (tray) was 15 times grain. This increase was due to the large uptake of water during germination of the grains. This action led to a sharply reduced in DM percentage in HBS