الفهرس 
Literature reviewOnly 14 pages are availabe for public view
 |  | from | 292 |  |  |
| | | from | 292 | | |
Abstract
This thesis consists of six parts:
Part I: General introduction
This part involves an introduction about hypertension disease, its symptoms and antihypertensive drugs including classifications and mode of action.
Part II: Literature review
This part includes a review of physical and chemical properties of the studied antihypertensive drugs, Azilsartan Medoxomil and Chlorthalidone as a first combination and Perindopril Arginine and Amlodipine Besylate as a second combination. Also, it includes different analytical methods reported in the literature that aimed for the determination of the selected drugs in pure form, mixtures and pharmaceutical formulation.
Part III: UPLC technique for determination of some antihypertensive drugs.
This part is divided into two sections:
Section A: Stability-indicating RP-UPLC method for simultaneous estimation of Azilsartan Medoxomil and Chlorthalidone in dosage forms and in presence of degradation products
The new developed UPLC method for the simultaneous determination of Azilsartan Medoxomil and Chlorthalidone is a green chemistry method. The wavelength detection for both drugs is 254nm and the mean recovery percentages are 100.39% ± 0.701 and 99.66% ± 0.969, respectively. Structure elucidation was done for the degradation products of both drugs.
Section B: Stability-indicating RP-UPLC method for the simultaneous estimation of Perindopril Arginine and Amlodipine Besylate in pharmaceutical formulation in the presence of their degradation products
The new developed UPLC method for the simultaneous determination of Perindopril Arginine and Amlodipine Besylate is a green chemistry method. The wavelength of detection for both drugs is 210 nm and the mean recovery percentages are 100.34% ± 0.958 and 99.15% ± 0.580 for PER and AML, respectively. Structure elucidation was done for the degradation products of both drugs.
Part IV: TLC-Densitometric method for the simultaneous estimation of Azilsartan Medoxomil and Chlorthalidone in solid dosage forms
TLC-densitometric method was proposed for simultaneous determination of Azilsartan Medoxomil and Chlorthalidone. The detection of Spots was carried out at the visible region after using 10% solution of phosphomolybdic acid in methanol as derivatizing gent. The mean recovery percentages for Azilsartan Medoxomil and Chlorthalidone are 99.56% ± 0.665 and 99.58% ± 0.963, respectively.
Part V: Spectroscopic determination of some antihypertensive drugs
This part is divided into three sections:
Section A: Spectrophotometric methods for the simultaneous estimation of Azilsartan Medoxomil and Chlorthalidone in solid dosage forms
Absorptivity factor method, Q absorbance ratio method and absorbance subtraction method were developed for determination of Azilsartan Medoxomil and Chlorthalidone. Azilsartan Medoxomil is measured at 300 nm and Perindopril Arginine is measured at 213 nm with the mean recovery percentage of (99.53% ± 0.798 and 99.26 ± 0.946), (99.67% ± 0.671, and 100.22 ± 1.014) and (99.61% ± 0.605 and 100.16 ± 0.989) for Azilsartan Medoxomil and Chlorthalidone by first, second and third method, respectively.
Section B: Spectrophotometric methods for the simultaneous estimation of Perindopril Arginine and Amlodipine Besylate in solid dosage forms
Absorptivity factor method, Q absorbance ratio method and absorbance subtraction method were developed for determination of Perindopril Arginine and Amlodipine Besylate was obtained. Perindopril Arginine is measured at 215 nm and Amlodipine Besylate is measured at 362 nm with the mean recovery percentage of (99.35% ± 0.794 and 99.80 ± 0.349), (99.24% ± 0.622% and 99.87 ± 0.590) and (99.21% ± 1.117 and 98.99 ± 0.738) for Perindopril Arginine and Amlodipine Besylate by first, second and third method, respectively
Section C: First derivative synchronous spectrofluorimetric method for the simultaneous estimation of Perindopril Arginine and Amlodipine Besylate in solid dosage forms
The first derivative synchronous fluorescence signals for Perinndopril Arginine and Amlodipine Besylate were measured at 280nm and 265 nm, respectively using wavelength difference Δλ = 10 nm. The mean recovery percentages for Perindopril Arginine and Amlodipine Besylate are 99.48% ± 0.778 and 100.42% ± 0.556, respectively.
Part VI: General discussion
This part includes a comparison between the different methods for determination of the four drugs and a comparison between the statistical analysis of the results obtained by the proposed methods and the reported method.
This thesis contains 128 references, 65 figures, 77 tables and ends with