الفهرس 
REVIEW OF LITERATURE PREVALENCE OF SEPSIS
HOSPITAL ACQUIRED INFECTION (HAI)Only 14 pages are availabe for public view
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Abstract
Background: Pediatric severe sepsis remains a public health problem. Sepsis
remains the most expensive condition in hospital stays. The delay in results
of conventional blood cultures may results in inappropriate treatment with
increased costs and delay in delivery of adequate treatment.
Objective: To determine the frequency of infection by selected organisms
including Candida species, Aspergillus species, MRSA, Acinetobacter
baumanni, E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa
among the pediatric patients admitted to pediatric intensive care unit with
sepsis by PCR, to compare PCR as detection methods with the conventional
blood culture methods and study sensitivity patterns of detected organisms to
antimicrobial therapy.
Methods: A cross-sectional observational study was carried out in Pediatric
ICU, Children Hospital, Ain Shams University. It included 45 critically ill
patients in pediatric age group (30days-18years) fulfilling the criteria of
sepsis. Details of admission and risk factors were recorded. Blood samples
were collected from each case for bacterial and fungal culture and sensitivity
and PCR for selected organisms.
Results: Total number of positive PCR was 30 in 23 patients with polymicrobial
infection in 6 patients (51.1%) compared to 10 for blood culture in
10 patients (22.2%). The frequency of MRSA was 16 (35.6%) of the studied
patients by PCR compared to (0%) by blood culture; E.Coli 3 (6.6%) by
PCR compared to (0%) by blood culture; Candida albicans 8 (17.8%) by
PCR while only 7 (15.6%) were detected by culture and 2 patients (4.4%)
with Acinetobacter and one (2.2%) with Klebsiella were detected by both
blood culture and PCR .
Conclusion: There was a disagreement between PCR and conventional blood
culture as regard the detection of both MRSA and E coli. PCR methods were
able to rapidly detect a wider panel of microorganisms and to determine the
presence of bacteria especially in those patients with prior antimicrobial
treatment.
Keywords: pediatric sepsis, MRSA, Candida albicans, E coli, Klebsiella,
Acinetobacter, PCR, PICU.