الفهرس 
HEPATOCELLULAR CARCINOMAOnly 14 pages are availabe for public view
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Abstract
In Egypt, liver cancer is the first most common cancer in male and is the second common cancer in females and it is the second cause of cancer mortality in both sexes.
Early detection of HCC is possible with ultrasound scanning and AFP monitoring, although the use of AFP as a screening test is complicated by frequent false positive and false negative results. The diagnostic sensitivity of AFP for detecting HCC in early stage is around 60% and specificity of 59.7%. Therefore, it highlights the need for new early detection biomarkers more useful and accurate for HCC.
In this current study, evaluation of exosomal mRNA-RAB11A, miRNA-1298 and lncRNA-RP11-583F2.2 expression in serum samples was performed by Real-Time PCR. Then the results were calculated and statistically analyzed by the SPS software.
The results of the current study revealed highly significant inverse correlation between serum exosomal miR-1298 and mRNA-RAB11A gene expression, also there was a highly significant positive correlation between exosomal lnc-RP11-583F2.2 and mRNA-RAB11A gene expression and also there was a highly significant inverse correlation between serum exosomal miR-1298 and lnc-RP11-583F2.2 based on fold change (RQ) among the three study groups, these results indicating that exosomal lnc-RP11-583F2.2 acting as competing endogenous RNA which targeting miR-1298.
Regarding HCC group, no significant correlation between fold change of serum mRNA-RAB11A, miR-1298 and lnc-RP11-583F2.2 expression and different laboratory parameters as AST, ALT, total bilirubin, direct bilirubin, albumin, INR and AFP.
In early stage of HCC, sensitivities of mRNA-RAB11A, miRNA-1298 and lncRNA-RP11-583F2.2 by quantitative PCR were 77.8%, 96.3% and 96.3% respectively, while the combined sensitivity of mRNA-RAB11A and lnc-RP11-583F2.2, mRNA-RAB11A and miR-1298, miR-1298 and lnc-RP11-583F2.2 by quantitative PCR in early stage of HCC were 98.3%, 100% and 100% respectively.
These findings supported our previous hypothesis that the exosomal mRNA-RAB11A, miR-1298 and lnc-RP11-583F2.2 might play an important role in early detection of HCC, and can be used as non invasive biomarkers to improve the sensitivity of HCC diagnosis.
So the findings of this study demonstrated that estimation of serum exosomal mRNA-RAB11A, miR-1298 and lnc-RP11-583F2.2 could have a potential diagnostic and screening target in patients with HCC in the future especially when used in combination.