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العنوان
CHEMISTRY AND BIOLOGICAL STUDY OF LEAVES EXTRACT OF PISTACIA ATLANTICA DESF.GROWING IN LIBYA \
المؤلف
OTHMAN, SALMA OTHMAN KALIFA.
هيئة الاعداد
مشرف / سالمة عثمان خليفة عثمان
مشرف / . ماهر عبد العزيز الحشاش
مشرف / سحر عوض الله محمد حسين
مشرف / عبدالرحمن حسن عبدالرحمن
تاريخ النشر
2019.
عدد الصفحات
190 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الكيمياء
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة عين شمس - كلية العلوم - الكيمياء
الفهرس
Only 14 pages are availabe for public view

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Abstract

The aim of the work is to search for new, potentially biologically active extract .The chemical and biological investigations of different extracts of pistacia atlantica desf.growing in Libya, resulted that the ethyl acetate extract of P. atlantica leaves contains a complicated mixture of phenolics, which includes mainly gallotannins as previously detected by 2D paper chromatographic screening. The isolation and identification of eleven polyphenoles (1-11),were elucidated for the first time from this plant ,Gallic acid (1) ; P-hydroxy benzoic acid (2) ; 1,2,3,4,6-penta-O-galloyl-ß-D-4C1- glupyranose (3) ;1, 6-di-O-galloyl-β-4C1- glucopyranose (4) ; 1,3-di-O-galloyl-β-4C1- glucopyranos(5) ; 2,3-di-O-galloyl-(α/β) -4C1- glucopyranose(6) ; 2,3,6-tri-O-galloyl-(α/β)-4C1-glucopyranose(7) ;2,3-di-O-galloyl-4,6-O-hexahydroxy diphenoyl -(/)-glucopyranose ,Tellimagrandin I (8);Gallic acid 3-O-β-4C1-glucopyranoside(9);Ellagic acid(10) ; 3, 3’-dimethoxyellagic acid (11).The structures of all isolated compounds were elucidated by conventional methods, spectroscopic analysis, including 1D and 2DNMR,ESIMS and HRESI mass as well. The search for new, potentially biologically active extract becomes much more efficient after identification of all compounds in that mixture. The in vitro antioxidant activity using the stable free radical DPPH (2,2-diphenyl-1-picrylhydrazyl) method of the extracts proved that ethyl acetate extract possesses a distinct radical scavenging effect at the different concentrations and the extract completely inhibited DPPH absorbance at a concentration 77 μl , the percentage of inhibition obtained 96.4% can be considered as a full absorbance inhibition of DPPH . Also, the IC50 of ethyl acetate extract of P. atlantica showed an IC50 value of 8.41±0.24 μg/ml compared with IC50 value of ascorbic acid 1.82±0.33 μg/ml. It could be concluded from the obtained results that the P. atlantica ethyl acetate extract shows a very high antioxidant capacity which is very close to the value of the reference standard used, which may be attributed to its high polyphenolic content. P. atlantica extracts . In vitro antibacterial activity of the extracts of P.atlantica leaves were studied against five bacteria strain and fungi . The tested organisms were Gram- negative bacteria (Escherichia coli ATCC-8739,Pseudomonas aerogenasa ATCC-9027), Gram-positive bacteria (Bacillus subtilis, ATCC-6633) and Staphylococcus aureus,ATCC – 6538) and unicellular fungi as (Candida albicans ATCC-90028), (Aspergillus niger ATCC-7966). The active ethyl acetate extract shown a sensitizing effect against Staphylococcus aureus , 20mm inhibition zone while the methanolic extract effect against Bacillus Subtilis was 19.5 mm inhibition zone.
The present study has revealed that Pistacia Atlantica desf. is capable of synthesizing and accumulating different types of phenolics, including mainly ellagitannins, gallotannins. The ethyl acetate extract showed a higher cytotoxicity to Colorectal adenocarcinoma cell line (Caco-2) with IC50 = 3.38 µg/ml and Prostate carcinoma cell line (PC3) with IC50=14.3 µg/ml. Furthermore, the methanol extract was least cytotoxic to normal cell lines. The strong cytotoxic potential was observed in pure compound(6) to all three cancer cell lines hepatocellular carcinoma (HEPG2), Colorectal adenocarcinoma cell line (Caco-2), Caucasian breast adenocarcinoma (MCF7), IC50 of HEPG2 value = 4.5 µg/ml., IC50 for Caco-2 was 11 µg/ml. and MCF7, IC50=13.5 µg/ml. as well.
The results indicate that the ethyl acetate extract of P. Atlantica growing in Libya is a promising source for bioactive compounds which exhibit potent cytotoxic activity and capable of synthesizing and accumulating different types of phenolics which were elucidated for the first time from this plant .