الفهرس 
MycotoxinsOnly 14 pages are availabe for public view
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Abstract
Aflatoxin B1 (AFB1) is one of the most potent naturally occurring
hepatic carcinogens to both human and animals. The present study aimed
to assess the effect of encapsulated mycotoxin binders (detoxifying
agents) inside yeast cell walls (YCW) to detoxify AFB1 in vitro using the
gastrointestinal models.
As yeast cell walls have been previously reported to bind AFB1.
The eight different yeast cell wall-based materials were tested in vitro for
AFB1 binding.
The results showed that Glucan Mannan Lipid Particles (GMLPs)
from Saccharomyces cerevisiae cell walls recorded the highest AFB1
adsorption 0.2 μg AFB1/mg GMLP in simulated gastric fluid (SGF) after
10 min, and 0.17 μg AFB1/mg GMLP in simulated intestinal fluid (SIF)
after 1h. GMLPs are hollow 3-4 micron porous microspheres that provide
an efficient system for the synthesis and encapsulation of AFB1-absorbing
nanoparticles (NPs).
Humic acid nanoparticles (HA-NPs) were synthesized within the
GMLP cavity by complexation with ferric chloride. The hybrid
(GMLP/HA-Fe NPs) synergistically enhanced AFB1 binding compared to
un encapsulated HA NPs and other AFB1 absorbing materials. As a result,
the hybrid GMLP HA-NP formulation synergistically enhanced AFB1
binding 1.6– 6.5 fold compared to individual GMLP and HA components
in SGF and higher than 9 fold in SIF. Cellular cytotoxicity studies
demonstrated that GMLP HA-NP-AFB1 complexes were stable in both
SGF and SIF and have the ability to detoxify AFB1 119-57 fold.
The current data of in vivo studies cleared that AFB1 at 80 mg/ kg
b.wt is multiorgans toxicants due to its adverse effects on liver and
kidney. The formulation (GMLP/HA Fe NPs) at level 0.5 mg/kg b.wt was effective in partially reversing mycotoxicosis and alleviating losses in
relation to the negative control.
Key Words
Yeast cell wall (YCW), aflatoxin b1 (AFB1), glucan mannan lipid particle
(GMLP), nanoparticles (NPs), microencapsulation, mycotoxin binders
(MB), humic acid (HA), in vitro gastrointestinal models, stability,
cytotoxicity and in vivo studies.
Name: Zeinab Khaled Saad Hamza
Thesis Title: Control of mycotoxin using nanopolymers prepared from
natural sources.
Research Place:
1. University of Massachusetts Medical School, Program in molecular
medicine Department, USA.
2. National Research Center, Food Toxicology and Contaminants
Department, Egypt.