الفهرس 
1. Obesity
Lipostatic Environmental factorsOnly 14 pages are availabe for public view
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Abstract
This study was carried out at Medical Research Centre,
Faculty of Medicine, Ain Shams University in the period
from March 2013 till June 2014 and included 30 obese subjects
and 30 non-obese controls.
The aim of this study was to determine the changes of
miRNA-143 expression in obesity compared to normal
individuals.
The studied individuals were divided into three main
groups:
group I: Healthy normal individuals with BMI between
18.5 to 24.9 (n=15, mean BMI=24±1 and mean
age=44±11).
group II: Overweight individuals with BMI between 25 to
29.9 (n=15, mean BMI=27.8±1 and mean
age=38±10).
group III: Obese individuals with BMI 30 or more (n=30,
mean BMI=42±11 and mean age=37±11).
All participants of this study went through complete
medical assessment including family history of chronic noncommunicable
diseases, pattern of physical activity, symptoms
covering various systems and general examination including
blood pressure measurements were done.Furthermore, anthropometric measurements were
performed for them. BMI was calculated as the ratio of the
weight and height squared (kg/m2) and assessment of BMI was
done using categories reported by WHO (2012). In addition,
laboratory investigations including fasting blood sugar and
complete lipid profile were done.
Finally, miRNA extraction from serum and adipose
tissue of all subjects was done. Reverse transcription of miR-
143 and comparative quantitation of its expression by using
Real-Time PCR were done for all samples. Then, the collected
data were statistically analyzed by the SPSS software.
The results of this study showed that there was high
expression of miR-143 in paired serum and adipose tissue
samples of the obese group compared to normal and overweight
groups indicating its upregulation in human obesity. There was
a highly significant positive correlation between fold change of
miRNA-143 expression in serum and BMI among the three
groups as well as a positive correlation between its expression
in tissue and BMI among them.
There was no significant correlation between miR-143
expression and any of the demographic data of the patients
assuming that it may be an independent biomarker and
therapeutic target in obesity.