الفهرس 
Introduction and Aim of workOnly 14 pages are availabe for public view
Abstract
Some miRNAs and their target genes have a great role as molecular markers for breast cancer diagnosis. A total of 150 women were enrolled in the present study and classified into three groups as 80 women diagnosed with primary breast cancer, 40 women with benign breast conditions and 30 normal healthy women. Candidate miRNAs (miRNA-17–5, miRNA-155 and miRNA-222) and their targeted genes (MMP-9, MMP-2 and TIMP3) respectively were studied in the three investigated groups, as a minimal noninvasive tool among early diagnosed breast cancer patients and their diagnostic efficacy were compared with tumor markers CEA and CA15.3 as two common tumor markers used in screening of breast cancer with a focus on their sensitivities and specificities among highly risk breast cancer patients. Relation between miRNAs and their targeted genes expression and clinicopathological characteristics and different histopathological subtypes and stages of breast cancer patients were assessed. In addition sensitivity, specificity, positive and negative predictive values (PPV, and NPV, respectively), and accuracy for miRNAs and their targeted genes were assessed. Also the diagnostic efficacy was detected by plotting ROC curve for all markers.
In conclusion, Candidate miRNAs (miRNA-17–5p, miRNA-155 and miRNA-222) and their targeted genes (MMP-9, MMP-2 and TIMP3) expressions in circulating blood samples can be a valuable molecular marker for detection of breast cancer preoperatively. Moreover, detection of these miRNAs and targeted genes using real time PCR technique was forefront than tumor markers (CEA and CA15.3) for early diagnosis of breast cancer especially to high-risk groups as those with early clinical stages and low graded tumors.