الفهرس 
RED BLOOD CELL PHYSIOLOGY
The Embden-Meyerhof PathwayOnly 14 pages are availabe for public view
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Abstract
During storage, RBCs undergo various metabolic,
structural and morphological changes, the so-called
storage lesions. The acceptable extent of the metabolic changes
forms a major part of the blood bank quality guidelines.
PRBCs are widely used for the treatment of patients with
anemia of different etiologies. For this purpose it is important
that the transfused RBCs preserve their metabolic capacity and
mechanical functions. It is therefore very important to control
the quality of PRBC units.
The method by which blood products are produced, the
storage solution used, and other factors as prestorage
leukoreduction have an effect on the final product and may
influence quality. Product standardization is thus a challenge
facing all blood biologic manufacturers
The current study was carried out on 20 PRBC units at 4
testing time points (D1, 10, 20 and 35) to evaluate the effect of
storage on PRBCs. We measured various biochemical
parameters ( Plasma Na+, K+, ATP , 2,3 DPG and blood gases)
and other hematological parameters (WBCs, Hb, Hct, %
hemolysis, RBC indices and abnormal cells %) in rder to
assess the quality of these units according to the regulatory
standards.the results of this study demonstrated that with increasing
the period of storage, there was statistically significant increase
in plasma K+ concentration, decrease in plama Na+
concentration, ATP and 2,3 DPG. Also, there was statistically
significant increase in PCO2 level, decrease in PO2 level and O2
saturation.
Regarding the hematological parameters measured there
was a statistical significant decrease in WBCs, increase in %
hemolysis, MCV and abnormal cells %.
On performing a correlation study, there was a moderate
significant correlation between the increase in % hemolysis and
the % decrease in WBC count at the end of storage (at D35)
and this is due to the degeneration of WBCs and releasing of
various chemicals and enzymes, especially proteases from the
leukocytes. Also, there was a moderate correlation between
increase % hemolysis and % decrease of ATP denoting the
effect of ATP depletion on Loss of membrane deformability
and viability.
Upon applying the WHO and FDA quality control
standards to our PRBC units across storage time, all of the units
were within the acceptable quality control standards except for
one unit (5%) was below the WHO guideline level as regards
Hb content. Also 4 units (20%) were below the WHO guideline
level for Hct level. Finally, 2 units (10%) exceeded the FDA.guideline level for % hemolysis at D1 and they increased to 3
units (15%) at D35.
Finally, our study demonstrates that the storage lesion
(biochemical and hematological changes) is a condition which
will always occurs with the prolonged storage time. Some
measures could be taken to slow down this process to improve
the quality of the PRBC units at different storage times.
It is our duty to properly apply the quality control
measures from the beginning of the process, starting from
selection of donors, processing (anticoagulant, additive solution
and blood bag material used) and storage conditions
(refrigerator temperature) in order to be able to determine the
good and sufficient units which will benefit the patients.