الفهرس 
Hepatocellular CarcinomaOnly 14 pages are availabe for public view
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Abstract
The present study was conducted to evaluate the efficiency of
biologically synthesized platinum nanoparticles (Pt NPs) in treatment
of hepatocellular carcinoma compared to cis-platin both in vitro and
in vivo. The treatment efficacy of Pt NPs was evaluated by measuring
antioxidant activities against oxidative stress caused by
diethylnitrosamine (DEN) in liver tissue. The measurements included
reduced glutathione (GSH) content and superoxide dismutase (SOD)
activity, as well as malonaldialdahyde (MDA) level. Liver function
tests were also determined, in addition to the evaluation of serum
alpha-fetoprotein (AFP), caspase-3 (Casp-3) and cytochrome c (Cyt
c) in liver tissue. Total RNA extraction from liver tissue samples was
also done for the relative quantification of B-cell lymphoma 2 (BCL-
2), matrix metallopeptidase 9 (MMP-9) and tumor protein p53 (P53)
genes. Histopathological examination was also performed for liver
tissue. Results showed that Pt NPs is more potent than cis-platin in
treatment of HCC induced by DEN in rats as it ameliorated the
investigated parameters towards normal control animals. These
findings were well appreciated with hispathological studies shown in
DEN group treated with Pt NPs, suggesting that Pt NPs can serve as
good therapeutic agents for the treatment of HCC which should attract
further studies.