الفهرس 
Introduction
Embryology of the liverOnly 14 pages are availabe for public view
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Abstract
Methotrexate is one of the drugs that are used in the treatment of many autoimmune and neoplastic diseases and the management of the patients with this drug may require a long period of time that exposes the patients to many side effects especially the hepatotoxicity.
The aim of the research is to study the effect of methotrexate injection on the histological structure of the liver of the adult albino rat and also the possible protective role of montelukast.
In the present study, 80 adult albino rats were used and they were divided into 4 groups; the first group (the control group). The second group (MLGroup) was given montelukast (Singulair tb4mg, 10 mg/kg daily for 10 days p.o.).The third group (Mtx Group)was given a single high dose of methotrexate by intraperitoneal injection (20mg/kg).
The fourth group(Mtx + ML Group) was given methotrexate (single dose 20 mg/kg i.p.) + montelukast (10 mg/kg daily for 10 days p.o., after 3 days methotrexate injection). 24 h after the last injection, rats in all groups were killed and the liver tissues of rats were collected for further analyses. The liver tissue specimens were placed in formaldehyde solution for histological and immunohistochemical examination by light microscopy.
The biochemical study demonstrated that there was increase in the serum levels of AST&ALT significantly in the methotrexate group. Montelukast significantly decrease these parameters.
The light microscopical study showed histological changes in the liver of the rats. These changes were represented by congestion of the central veins and the blood sinusoids and also congestion of the portal veins.
The hepatocytes were also exposed to degenerative changes in the form of the appearance of vacuoles in the cytoplasm; pyknotic nuclei and apoptosis of some cells. There was also inflammatory cell infiltration.