الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Fluorescence spectroscopy has many advantages making
it an appealing choice for low concentrations samples analysis.
Fluorometry may achieve limits of detection several orders of
magnitude lower than those of most other techniques. This is
known as the fluorescence advantage .fluorescence
measurements are rapid and inexpensive, Because of the low
detection limits, so fluorescence is widely used for
quantification of trace constituents of biological and
environmental samples.
The study is divided into four chapters:
Chapter one
Introduction and literature review: This
Chapter includes a general introduction on fluorescence
spectroscopy and its advantage in modern analytical techniques
and its theory about sensing and different types of fluorescent
probes. A literature review on the measurement of enzymes
such as glucose oxidase and 3-nitrotyrosine using different
method of detection. Recent references related to research has
been used.
Summary
Chapter two
Describes a highly selective and sensitive spectrofluorimetric
method for the assessment of 3-nitrotyrosine (3NT) in Serum
Samples based on the quenching of luminescence intensity of
the Optical sensor Pd-complex.
It was found that there is energy transfer between photo probe
and 3-nitrotyrosine in addition to measurement of photo probe
emission at exication wave length (λEX =350 nm) under
different condition of solvent and pH.
The optimum conditions for measurement are DMF as
solvent and pH=10.5 photo probe has been used for 3-
nitrotyrosine concentrations by quenching of the photo
probe(Pd-complex ) band at wave length of emission (λem=425
nm) after adding different concentration of 3-nitrotyrosine
fluorescence intensity under optimum condition and making
linear relation between fluorescence intensity and 1/different
concentration and making wide linear working range (1.42 x
10−9 to 3.8 ×10−6 mol L-1 ) [3NT] with a correlation coefficient
of 0.998 and a detection limit of 2.98 × 10−9 mol L-1.
Thus, the use of the photo probe by measuring and estimating
3-nitrotyrosine in different samples of serum to the many of
liver disease patients.
Summary
Chapter three
Describes a highly selective and sensitive spectrofluorimetric
method for the assessment of glucose oxidase [GO] in Serum
Samples based on the quenching of luminescence intensity of
the Optical sensor Pd-comlex.
It was found that there is energy transfer between photo probe
and H2O2 (which result from react β-D glucose + H2O + O2 in
presence glucose oxidase enzyme) in addition to measurement
of photo probe emission at exication wave length (λex =450 nm)
under different condition of solvent and pH.
The optimum conditions for measurement are acetonitrile as
solvent and pH=9.5. photo probe has been used for GO
concentrations by quenching of the photo probe band at wave
length of emission (λem=530 nm) after adding different
concentration of uric H2O2 fluorescence intensity under optimum
condition and making linear relation between fluorescence
intensity and 1/different concentration and making wide linear
working range (1.0 × 10−9 to 5.0 ×10−6 mol L−1 (r = 0.998). And
The detection limit (S:N = 3) (LOD) is 2.8 x 10-10mol L−1 ..
Thus, the use of the photo probe in the enzyme glucose oxidase
activity estimate by measuring and estimating H2O2 in different
samples of serum to the many diabetes patients.
Summary
Chapter four
The efficiency of excited-state interaction between Eu3+ doped
in sol- gel matrix and the industrial product ciprofloxacin of
(CFX) has been studied in different solvent and pHs. A high
luminescence intensity peak at 617 nm of europiumciprofloxacin
complex at λex=365 nm in acetonitrile was
obtained. The photophysical properties of the red emissive
Eu3+ complex doped in sol-gel matrix have been elucidated, the
europium was used as optical sensor for the assessment of
ciprofloxacin in the pharmaceutical tablets and serum samples
at pH 8.0 and λex = 365 nm with a concentration range of 5.0
×10−9 - 1.0 ×10−6 m ccxol L−1 for ciprofloxacin, correlation
coefficient of 0.99 and detection limit of 1.65 ×10−9 mol L−1.