الفهرس 
Fluorescence spectra propertiesOnly 14 pages are availabe for public view
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Abstract
Fluorescence spectroscopy has many advantages making it an appealing choice for low concentrations samples analysis. Fluorometry may achieve limits of detection several orders of magnitude lower than those of most other techniques. This is known as the fluorescence advantage .fluorescence measurements are rapid and inexpensive, because of the low detection limits. So fluorescence is widely used for quantification of trace constituents of biological and environmental samples.
The study is divided into four chapters:
Chapter one (Introduction and literature review)
Includes a general introduction on fluorescence spectroscopy and its advantage in modern analytical techniques and its theory about sensing and different types of fluorescent probes. A literature reviews the different metods that can used for the measurement of glucose oxidase enzyme, gatifluoxacin and TSH in real samples Chapter two
Deals with the different experimental techniques that were employed to study the structure, spectral characterization of photo probe of (2,6-dimethylbenzyl)amino)((1E,2Z)-2-(2-(2,6-dimethylphenyl)-1-oxo-1H-pyrrolo[3,4-b]quinolin-3(2H)-ylidene)2(2,6dimethylphenyl)amino)1(2,6dimethylphenyl)imino)ethyl)palladium(III) chloride to detect hormone TSH and deals with the experimental results obtained from the structural and spectral characterization of photo probe and the selected hormone like (TSH).
Also discuss the experimental data of the structural characterization of photo probe studying absorption spectrum of photo probe with the addition of different concentration of TSH enzyme. It was found that there is energy transfer from TSH to photo probe in addition to measurement of photo probe emission at excitation wave length (λex =390 nm) under different condition of solvent and pH=9.5. It was also found that the optimum conditions for measurement are DMSO as solvent and pH=9.5 . The photo probe (I) has been used for TSH concentrations by the enhancement of photo probe band at wave length of emission (λem=486 nm) after adding different concentration of TSH fluorescence intensity under optimum condition and making linear relation between fluorescence intensity against inverted concentration and making wide linear working range 1.9 x 10-10 IU/L to 7.99x10-8 IU/L, (r = 0.996). The detection limit is 0. 60 x 10-9IU/L and the limit of quantification (LOQ) is 1.9 × 10-9IU/L .The photo probe has been used in different serum samples.
Chapter three
Deals with the different experimental techniques that have been employed to study the structure, spectral characterization of photo probe (Bis(dialkylamino)phenoxazinium) to detect glucose oxidase and deal with the experimental data of structural characterization to study the absorption spectrum of photo probe in addition of different concentration of H2O2. It was found that there is energy transfer between photo probe and H2O2 in addition to measurement of photo probe emission at exication wave length (λex =310 nm) under different condition of solvent and pH .The optimum conditions for measurement are DMSO as solvent and pH=7.3 photo probe was used for H2O2 concentrations by enhancement of the photo probe(II) band at wave length of emission (λem=367 nm) after adding different concentration of H2O2 fluorescence intensity under optimum condition and making linear relation between fluorescence intensity and different concentration and making The method is used in liner range from 7.0 x 10-11mol/L to 9.08 x 10-9mol/L, (r=0.998) and Limit of detection (LOD) is 3.91 x 10-8mol/L.
The photo probe has been used in different samples like serum.
Chapter four
Describes a highly selective and sensitive spectro fluorimetric method for the assessment of gatifloxacin in pharmaceutical tablets and serum sample based on the enhancement of luminescence intensity of the optical sensor Tb3+ ion. In ammonia buffer solution (pH = 8.0) the gatifloxacin can remarkably enhance the luminescence intensity of Tb3+ ion in Tb3+- GTX complex at λex= 340 nm. The produced luminescence intensity of Tb3+- GTX complex in acetonitrile is proportional to the concentration of CFX and can be used as optical sensor for its determination Limit of detection (LOD)= 1.6 x 10-9mol/ L.