Search In this Thesis
   Search In this Thesis  
العنوان
Effect of Isoflavones on Vagina of Senile Albino Rats:
المؤلف
Ibrahim, Nesma Abdelhady Hassan.
هيئة الاعداد
باحث / Nesma Abdelhady Hassan Ibrahim
مشرف / Hemmat Abdelkader Abdelhamid
مشرف / Rania Ahmed Salah El-Din
مناقش / Enas Anwar Bekheet
تاريخ النشر
2018.
عدد الصفحات
111 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
تشريح
تاريخ الإجازة
1/1/2018
مكان الإجازة
جامعة عين شمس - كلية الطب - قسم التشريح وعلم الاجنة
الفهرس
Only 14 pages are availabe for public view

from 111

from 111

Abstract

M
enopause is the age of cessation of menses in females. It is either natural (at age of 45-50 usually) or induced by ovariectomy. It’s accompanied by a variety of symptoms usually due to estrogen deficiency; one of them is the urogenital. Concerning the vagina, dryness and itching, were the most irritating in females after menopause. These symptoms were due to the deprivation of estrogen, and therefore some women tend to take estrogen replacement therapy to relieve these symptoms. Recently, it was found that estrogen replacement therapy has hazards of carcinogenic effects especially for breast cancer, and so it became necessity to search for non-estrogen replacement therapy for postmenopausal manifestation.
Soy isoflavones are groups of phytoestrogens that have the effect of estrogen. It is found that phytoestrogen had a great effect in relieving postmenopausal manifestations such as hot flushes, osteoporosis, coronary heart diseases, and psychiatric disorders associated with menopause.
The present study was performed to detect the effect of phytoestrogens in decreasing the vaginal dryness and atrophic changes in senile rats and whether they can be used as a treatment of these atrophic changes.
Twenty-four female albino rats were used in this study, 16 of them about 24 months weighing about ”250-300 gm”, while 8 were about 6 months and weighing about ”100-150 gm”. Rats were divided into three groups. The first group was the group that aged 6 months and served as adult control group which was given vehicle. The second group served as senile control that received vehicle also. The third group included aged female rats that received isoflavones therapy for two weeks. Rats from the three groups were sacrificed and the vaginae were excised and processed. All vaginal specimens were subjected to light microscopic examination after staining by H&E, Mallory Trichrome stain, PAS Stain, and immunohistochemistry stain for estrogen receptor α.
The vagina of the adult female rats was formed of keratinized stratified squamous epithelium resting on highly cellular lamina propria that is infiltrated with lymphocytes. The basal layer of the epithelium was formed of columnar cells with oval nuclei. The lamina propria showed dense collagen fibers. The epithelium contained high glycogen that concentrated mainly in the superficial layers of the epithelium. Immunohistochemistry showed that the vaginal epithelium has positive reaction for the presence of estrogen receptor subtype alpha.
The vagina of senile rats showed thin non keratinized epithelium which had lost the regular papillary appearance with apparent decrease in the number of layers and focal areas of attenuation. The cells showed signs of degeneration in the form of vacuolated cytoplasm, pyknotic and fragmented nuclei. The surface epithelium lacked keratin. The lamina propria showed distorted and widely separated collagen fibers and there was distortion of the regular arrangement of muscle fibers. The epithelium also showed lack of the glycogen content through the apparent negative reaction to PAS stain. There was also lack of estrogen receptors in the epithelium which explained the other histological findings.
The vagina of senile rats which received isoflavone treatment showed marked increase of the thickness of the epithelium with normal architecture as that of the control group. Cells showed disappearance of the cellular degeneration. The superficial layer showed restoration of the keratinization. The lamina propria showed dense collagen fibers and restored its cellularity and vascularity. The muscle layer restored its regular arrangement and its thickness. The epithelium also showed restoration of its glycogen content and promotion of the number of estrogen receptors apparently more than even that of the adult vagina.