الفهرس | Only 14 pages are availabe for public view |
Abstract Bifidobacterium spp is normally found in the human intestines and characterized by gram-positive, catalasenegative, non-sporeforming, non-motile. Bifidobacterium spp are referred as probiotics due to their antimicrobial activities and human health benefits. The most important characteristic of Bifidobacterium infantis is affecting tryptophan metabolism. Serotonin synthesis in the brain depends on availability of its precursor tryptophan. Since interruption in the synthesis of serotonin plays a key role in physiology of depressive disorders. A deficiency of Bifidobacterium infantis may contribute to depression disorders as well. Increased Reactive oxygen species (ROS) production may lead to changes in membrane viscosity may affect serotonergic receptor functions. So, it is inviting to hypothesize that these relationships support a possible association between oxidative stress and depression. The aim of this study was to evaluate the antidepressant and antioxidant effect of Bifidobacterium infantis in management of depression state which correlated with oxidative stress. The effect was assessed by measuring whole blood serotonin, serum malondialdehyde, serum nitric oxide and whole blood glutathione peroxidase levels. 100 healthy male albino rats were divided into five groups 20 rats in each.group (I): Control group: consisted of 20 rats that not exposed to forced swim test and didn’t suffer from any stress. group (II): Depression group: 20 rats that exposed to forced swim test daily for 14 days without any treatments. group III: Prophylactic group: 20 rats that have treatment dose of 1X1012 of Bifidobacterium infantis and 2 hours later they exposed to forced swim test daily for 14 days. Treatment groups: group (IV): probiotic treatment: 20 rats that exposed to forced swim test every day for 14 days (and then treated by 1X1012 of Bifidobacterium infantis for another 14 days. group V: SSRI treatment: 20 rats that exposed to forced swim test every day for 14 days and then treated with 10mg/5ml of citalopram for another 14 days Note: group IV and group V have before and after treatments results and were called treatments groups) Blood samples were collected from all subjects for determination of the different parameters. The study revealed that there was a significant reduction in whole blood serotonin level, a significant increase in serum malondialdehyde and serum nitric oxide and a highly significant decrease in whole blood glutathione in all rats that gain depression by forced swim test for 14 days (group II, group IV before, group V before) On the other hand, oral administration of Bifidobacterium infantis for 14 days as a prophylactic treatment (group III) showed no significant difference in measured parameters except for glutathione peroxidase which showed a highly significant increase when compared with control. But, there was a highly significant difference between group III (prophylactic treatment) and group II (depressed group) Oral administration of Bifidobacterium infantis for 14 days in group IV (treatment) showed no significant difference in all measured parameters when compared to control. But, there was a highly significant difference in all measured parameters when compared to group IV before treatment. On the other hand, Oral administration of citalopram for 14 days in group V showed no significant change in measured parameters when compared to group V before treatment except for serum malondialdehyde which showed a highly significant difference before and after treatment. from the previous data it is obvious that Bifidobacterium infantis treatment has got significant antidepressant and antioxidant activity in management of depression state which correlated with oxidative stress. |