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العنوان
STUDIES ON SALMONELLA INFECTION IN CHICKENS AND IT’S RELATION TO THE MOLECULAR charACTERS /
المؤلف
Mosalem, Shimaa Hassan Ahmed.
هيئة الاعداد
باحث / شيماء حسن احمد مسلم
مشرف / محسن محمد زكى الدمرداش
مناقش / محمد حسين حسن عواض
مناقش / محمد السيد عنانى
الموضوع
Salmonella infections in poultry. Salmonella infections.
تاريخ النشر
2017.
عدد الصفحات
177 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
22/7/2017
مكان الإجازة
جامعة قناة السويس - كلية الطب البيطري - طب الطيور والأرانب
الفهرس
Only 14 pages are availabe for public view

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Abstract

Salmonella is considered one of the most threatened bacterial pathogen to the poultry industry with health risk impact on human health. The pathogen causes sever economic losses in poultry farms. Salmonella recently developed multi drug resistant (MDR) against several antimicrobial agents, which have potential risk on human health as much as the pathogen can infect human.In our study Salmonellae was detected in 5.7% of examined samples and Salmonella were serologically typed as S. Enteritidis, S. Kentucky, S. Kralingen, S. Fillmore , S. Hindmarsh, S. lagos, S. Birkenhead, S. Dublin and untyped strains were recorded with a percentage of (11.8%, 11.8%, 11.8%, 11.8%, 5.9%, 5.9%, 5.9%, 5.9% and 5.9% respectively) . S. Typhimurium was the most prevalent one with a percentage of (23.5%). Molecular detection of some antibiotic resistant and virulence genes showed that : eight out of eight invA gene was detected in 100% follow by Sul1 gene ( 62.5%) , blaTEM gene (50%) , tetA and aac(6′ )-Ib-cr (37.5%) ,aadB, dfrA, mph(A) and ereA (12.5%) . stn and sopB virulence genes were detected in most Salmonella serotypes in a percentages of 62.5% . Presence of Class 1 Integron in a percentage of 75% MDR Salmonella isolates. All serovars in this study showed highest resistance to Erythromycin, Ciprofloxacin and Trimethoprim / Sulfamethoxazole. High rates of multi drug resistance and of the presence of Integron found among the Salmonella isolates in this study suggests that legislation to enforce a more prudent use of antibiotics in both human and veterinary medicine should be implemented by the authorities in Egypt . The results of gyrA sequencing revealed that mutations in the gyrA gene were known to be the main resistance mechanism to quinolones. S. Enteritidis and S. Typhimurium (isolated from chicken) showed single mutations in the codons at the position 83 which changed from Serine (S) (standard protein) to tyrosine (Y) (mutated protein) in case of S. Enteritidis but from Serine (S) (standard protein) to Phenylalanine (F) (mutated protein) mutations in gyrA gene of S. Typhimurium was recorded in this study .