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العنوان
The Role Of Sirtuin 1 in Diabetic Nephropathy /
المؤلف
EL-Ghandour, Haidi Shebl Ahmed.
هيئة الاعداد
باحث / Haidi Shebl Ahmed EL-Ghandour
مشرف / Shadia Abd El-Hamid Fathy
مشرف / Tahany M. Abd El-Moneam
مناقش / Wafik A.El-Khayat
تاريخ النشر
2017.
عدد الصفحات
148p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Biochemistry
تاريخ الإجازة
1/1/2017
مكان الإجازة
جامعة عين شمس - كلية العلوم - الكمياء الحيوية
الفهرس
Only 14 pages are availabe for public view

from 148

from 148

Abstract

Summary
Diabetic nephropathy (DN) is a serious complication of diabetes mellitus; it initially manifests with microalbuminurin and progresses towards end-stage renal failure. Sustained diabetes-related metabolic and haemodynamic perturbations can induce subclinical low-grade renal inflammation and drive kidney from repair response to damage process, eventually to renal fibrosis (Shirong et al., 2016).
The aim of the present study is to investigate the relationship between serum situin 1 and advanced glycation end products (AGEs) in Egyptian patients with type II diabetes and patients with diabetic nephropathy.
Diabetic patients and healthy control individuals (100 males and females) were fully studied by means of clinical and laboratory examinations before being selected for this study. The groups divided as the following:
group (I) which contains 20 healthy individuals, group (II) contians 30 normoalbuminuric patients, group(III) includes 50 patients with diabetic nephropathy, group (IIIa) contains 30 patients with microalbuminuria and group (IIIb) contains 20 macro- albuminuric patients.
The following parameters are estimated: fasting plasma glucose, insulin, HOMA-IR, totalcholesterol, triglycerides, HDL-C, LDL-C, urea, creatinine, blood urea nitrogen, AGEs, microalbumin and serum sirtuin-1.
The results can be summarized as following:
1. FPG showed a highly significant increase (p<0.001) in all patients compared to controls, while it showed a non-
Summary
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significant difference between microalbuminic and macroalbuminuric patients.
2. Insulin level showed a highly significant decrease (p<0.001) in normoalbuminuric patients when compared to controls, but it showed a non-significant difference in micro and macroalbuminuric patients when compared to controls.
3. Homeostasis model assessment for insulin resistance (HOMA-IR) showed a highly significant increase (p<0.001) in diabetic patients with nephropathy compared to controls
4. Total cholesterol levels showed a highly significant increase (p<0.001) in all patients compared to controls. In addition, it showed a non-significant elevation in microalbuminuric and macroalbuminuric patients compared to normoalbuminuric group.
5. Triacylglycerols level showed a highly significant elevation (p<0.001) in all diabetic patients compared to controls. While it showed a non-significant difference between microalbuminuric, macroalbuminuric and normoalbuminuric groups.
6. HDL-C showed a highly significant decrease (p<0.001) in all diabetic patients compared to controls.
7. LDL-C showed a highly significant increase (p<0.001) in all diabetic patients when compared to controls .While it showed no significant difference in microalbuminuric and macroalbuminuric patients compared to normoalbuminuric group.
Summary
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8. Urea showed a highly significant increase (p<0.001) in diabetic patients with nephropathy compared to controls and showed a significant elevation (p<0.001) in microalbuminuric and macroalbuminuric patients compared to normoalbuminuric group.
9. Creatinine showed a highly significant increase (p<0.001) in only diabetic patients with nephropathy when compared to the control group.
10. Serum SIRT-1 levels showed significant decrease (p<0.05) in normoalbuminuric patients when compared to controls, while it showed a non-significant difference between normoalbuminuric ,microalbuminuric and macroalbuminuric patients.
11. Serum AGEs levels showed a highly significant elevation (p<0.001) in all diabetic patients when compared to the control group. But, it showed a non-significance increase in microalbuminuric patients and macroalbuminuric patients when compared to normoalbuminuric group.
12. Urinary microalbumin showed a highly significant increase (p<0.001) in diabetic patients with nephropathy when compared to controls and normoalbuminuric group.
In conclusion, serum Sirt1 level may be changed during the minimal renal impairment in the course of DM. Also, serum AGEs level is correlated with serum Sirt1 in diabetic nephropathy patients.