الفهرس 
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Abstract
An attempts aimed to join the good efforts of the team of biological control being done in Egypt for controlling the lepidopterous pest S. littoralis, 4 B. thuringiensis isolates have been isolated from four governorates in Egypt and subjected to characterization and identification by bioassay and PCR technology, using specific primers to amplify the whole open reading frame of the cry1A genes. Plasmid, protein patterns and RAPD PCR have been made to the isolates to offer a characteristic fingerprint for the B. thuringiensis isolates of interest.
The insecticidal crystal protein gene from field collected B. thuringiensis isolate 4 was cloned into one shot top 10 competent cell through pCR2.1 vector, and this step was followed by cry1A gene sequencing, where the gene alignment revealed the presence of a suspected point mutation.
This point mutation in the cry1Ac gene-as proved by the gene alignment- could be the direct purpose of the high mortality level caused by the isolate of interest.
Our attempts are to use this novel gene for further studies in order to introduce this gene in GMOs (Genetically Modified Organisms).
Keywords:
Bacillus thuringiensis, cry1A gene sequencing, RAPD PCR, cry1Ac, Cloning.