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العنوان
Mechanism of Disintegrin/like domain in Mesenchymal Stem Cells Homing to Mice Liver Injury Model /
المؤلف
Darwish, Amal Said Mohammed.
هيئة الاعداد
باحث / أمل سعيد محمد درويش
مشرف / هناء الطيب ناصر
مشرف / محمد فريد الاسمر
مشرف / أيمن رجاء بشير
مشرف / وليد سعيد زكي
تاريخ النشر
2017.
عدد الصفحات
214 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Biochemistry
تاريخ الإجازة
1/1/2017
مكان الإجازة
جامعة عين شمس - كلية الطب - الكيمياء الحيوية الطبية والبيولوجيا الجزيئية
الفهرس
Only 14 pages are availabe for public view

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Abstract

This study was done at the Research Unit of Natural Toxins, Medical Biochemistry Department, Ain Shams University Faculty of Medicine. Experimental design was assigned to include5 groups of white albino mice.
The aim of this study was to assess the molecular mechanism of disintegrin/ like domain on labeled bone marrow mesenchymal stem cell recruitment & homing into CCl4 induced injured liver and evaluate its effect & stem cell ability to regenerate functioning hepatocytes through comparative measuring of liver functions (serum ALT, AST, and serum albumin levels) as well as relative quantitative assessment of gene expression to the followings (stromal derived factor-1α(SDF-1α), hepatocyte growth factor (HGF), transforming growth factor beta 1 (TGF-β1) and CXCR4 genes) using real time PCR.
The studied groups of mice were classified into 5 main groups as follow:
group 1: Injected intra-peritoneally with normal saline as control once weekly for 3 weeks in a dose of 0.8 ml/kg body weight then sacrificed one week later.
group 2: Injected intra-peritoneally with CCl4 0.8 ml (95%)/kg body weight once weekly for 3 successive weeks then sacrificed one week later.
group 3: Injected intra-peritoneally with CCl4 0.8 ml (95%)/kg once weekly for 3 successive weeks followed one week later by intra-peritoneal injection of disintegrin/ like domain 0.3 mg/kg once weekly for 2 successive weeks then sacrificed one week later.
group 4: Injected intra-peritoneally with CCl4 0.8 ml (95%) /kg once weekly for 3 successive weeks followed one week later by intra-venous injection of labeled BM- derived stem cells at a concentration of 2 ×106 cells/ml (0.5 ml was injected) (cells were injected intravenously into mice tail vein)once then sacrificed 3 weeks later.
group 5: Injected intra-peritoneally with CCl4 0.8 ml (95%) /kg once weekly for 3 successive weeks followed one week later by intra-venous injection of labeled BM- derived stem cells 2 ×106 cells/ml (0.5 ml was injected) (cells were injected intravenously into mice tail vein)and intra-peritoneal injection of disintegrin/ like domain 0.3 mg/kg then sacrificed 3 weeks later.

All groups were subjected to
 Estimation of serum ALT, AST & albumin.
 Evaluation of (stromal cell-derived factor-1α (SDF-1α), hepatocyte growth factor (HGF) and transforming growth factor beta 1 (TGF-β1) genes) expressions by relative quantification REAL TIME PCR.
 Evaluation of labeled stem cells homing using fluorescent microscope for detection (group 4, group 5).
 Histo-pathological examination of H&E stained liver tissue using light microscope.
Then the results were calculated and statistically analyzed by the SPSS software and the outcome revealed the possible mechanism by which disintegrin/ like domain could enhance stem cells migration as well as regenerative effect of both where it showed improvement of the entire measured parameters (biochemically & histo-pathologically), suggesting the beneficial role of disintegrin/ like domain on mesenchymanl stem cells in management of liver injury.
The best findings were belonged to the group treated with both stem cells and disintegrin/ like domain after CCl4 liver injury than groups treated with disintegrin/ like domain or stem cells separately.
Further studies are recommended on larger scale to ensure and clarify the underlying mechanism by which disintegrin/ like domain could influence stem cells homing using different doses, concentrations, and durations.