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العنوان
Detection and Genotyping of Methicillin Resistance Staphylococcus aureus Infection and Colonization of Surgery Patients \
المؤلف
Abd El-Ghafar, Hisham Abd El-Aziz Ahmed.
هيئة الاعداد
باحث / Hisham Abd El-Aziz Ahmed Abd El-Ghafar
مشرف / Ahmed Osman Mostafa
مشرف / Mervat Ismail El Borhamy
مشرف / Adel Fahmy Hamed
تاريخ النشر
2017.
عدد الصفحات
260 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الصيدلة
تاريخ الإجازة
8/1/2018
مكان الإجازة
جامعة عين شمس - كلية العلوم - الميكروبيولوجي
الفهرس
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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major health hazards responsible for a large number of nosocomial (hospital acquired) infections worldwide and became of greater public health concern since the emergence of community acquired MRSA.
Out of 338 Staphylococcus aureus isolates collected from 374 patients, 108 (32.2 %) showed positive growth on Oxacillin Resistance Screening Agar Base (ORSAB) selective media for MRSA while only 105 (31.1%) isolates showed resistance to 30 µg cefoxitin susceptibility test and classified as MRSA. Among the MRSA isolates (105), 77 (73.3%) were identified as community acquired (CA) MRSA and 28 (26.7%) isolates were hospital acquired (HA) MRSA.
The frequency of resistant and susceptibility towards 19 antibiotics revealed that all MRSA (100%) isolates were sensitive to vancomycin (VA 30 µg) and linezolid (LZD 30 µg).
All of CA-MRSA and HA-MRSA isolates were screened for mecA, femA, femB, lukS-PV and lukF-PV (PVL) genes and intgrons class I and class II genes. Both mecA and femA genes were present in all (100%) HA-MRSA and CA-MRSA isolates. femB gene was recovered from all HA-MRSA isolates and 72 (93.5%) of CA-MRSA isolates.
PVL gene was detected in 8 (28.6 %) HA-MRSA isolates and seventy-one (92.2%) CA-MRSA.
Class I integron gene was recovered from 17 (60.7%) HA-MRSA isolates and 29 (37.7%) CA-MRSA isolates, while class II integron gene was recovered from only 3 (10.7%) HA-MRSA isolates and from five (6.5 %) CA-MRSA isolates.
Decolonization measures were applied on all colonized sites as per CDC, (2007) recommendation and the results of successful decolonization were 90.9% from nasal, 93.9% from groin and 80% from axilla and the total percentage of 91.8% successful decolonization was achieved.