الفهرس 
Colorectal CancerOnly 14 pages are availabe for public view
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Abstract
STUDY OF SERUM PROTEOMIC PATTERNS IN PATIENTS WITH PRIMARY COLORECTAL CANCER BASED ON MAGNETIC BEAD SEPARATION AND MATRIX ASSISTED LASER DESORPTION/IONIZATION- TIME OF FLIGHT MASS SPECTROMETRY (MALDI-TOF MS)
Eman abd-Elmonem Elgohary, Sayeda Abd El-Rahiem,Pacint El-Sayed Moez Adel Ahmed Elazab Elged , Amira Ibrahim Hamed, Suzan Eid Elshishtawy Ibrahim
Clinical pathology department, Ain Shams University.
ABSTRACT
Background: At present, the diagnosis of colorectal cancer (CRC) requires a colorectal biopsy which is an invasive procedure. We undertook this pilot study to develop an alternative method and potential new biomarkers for diagnosis, and validated a set of well-integrated tools called ClinProt to investigate the serum peptidome in CRC patients.
Methods: serum from 70 patients diagnosed with CRC by histological diagnosis, and 70 healthy volunteers were collected. Division was into a model construction group and an external validation group randomly. The present work focused on serum proteomic analysis of model construction group by ClinProt Kit combined with mass spectrometry. This approach allowed construction of a peptide pattern able to differentiate the studied populations. An external validation group was used to verify the diagnostic capability of the peptidome pattern blindly. An immunoassay method was used to determine serum CEA of CRC and controls.
Results: The results showed 65 differential peptide peaks in CRC, and health volunteers. A genetic algorithm was used to set up the classification models. five of the identified peaks at m/z 6686,6442, 3294, 2130 and 7843 were used to construct peptidome patterns, achieving an accuracy of 100% (> CEA, P < 0. 05). Furthermore, the peptidome patterns could differentiate the validation group with high accuracy close to 100%.
Conclusions: Our results showed that proteomic analysis of serum with MALDI-TOF MS is a fast and reproducible approach, which may provide a novel approach to screening for CRC.
Keywords: peptidome patterns - colorectal cancer - diagnosis - magnetic beads - MALDI-TOF mass spectrometry.