الفهرس 
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Abstract
Vibriosis is a globally threatening bacterial disease affecting mariculture with high mortalities and severe economic losses. Isolation and Identification of different vibrio species were performed to a total number of one hundred moribund and freshly dead Solea aegyptiaca, Epinephelus marginatus and Mugil cephalus collected from Lake Qarun and Gulf of Suez. Phenotypic picture and molecular identification based on use of 16SrRNA gene sequence confirmed 44 strains as vibrio species. Further molecular identification of retrieved vibrio spp. using species specific primers for collagenase, ToxR, Vvh and VFlu genes categorized 10 isolates belong to V.alginolyticus, 8 isolates belong to V.parahaemolyticus, 6 isolates belong to V.vulnificus and 20 isolates belong to V.fluvialis. where the total prevalence of vibriosis was 44 %. Formalin killed whole cell bacterin and Heat Killed whole cell bacterin were used for fish immunization as a control method of vibriosis and showed RPS% of 100 % and 27.2% respectively.