الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
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Abstract
Salmonellosis is one of the most infectious diseases in both human and animal which cause highly economic losses in poultry production, so attention must be paid for protection from this infection and rapid detection method of infection such as: PCR. Salmonella Enteritidis is considered the most cause of salmonellosis in broiler chicken and lead to human food poisoning out breaks. Chicken eggs and meat are considered the main source of Salmonella Enteritidis to human. The current study aimed to determine diagnostic value of PCR in detection of β-lactamase-producing Salmonella Enteritidis from broiler chicken and their susceptibility to different antibiotics groups also , In vitro assessment of β-lactamase-producing Salmonella Enteritidis on sensitivity of Staph. aureus to penicillin . A total of 210 tissue swabs (liver, intestine, spleen and gall bladder) were collected from broiler chickens suffering from diarrhea. The collected swabs were separately soaked into sterile buffered peptone water then inoculated in Rappaport Vassiliadis broth, and then plated onto XLD agar medium. The suspected colonies were picked up, purified and identified biochemically and morphologically. The results are summarized in the following points: Out of 210sampleswere examined and yielded 5 positive Salmonella isolates at percentage of 2.4 %. The 5 positive Salmonella isolates were further confirmed using PCR assay based on invA gene. Using PCR for detection of serotype of Salmonella Enteritidis based on sefA gene , 4 isolates out of 5 Salmonella isolates were Salmonella Enteritidis which representing 1.9% of total 210 examined samples and 80% of 5 positive Salmonella isolates . Results of PCR amplification for blaTEM gene antibiotic resistant gene of Salmonella Enteritidis revealed that 2 out of 4 obtained isolates of Salmonella Enteritidis were positive for blaTEM at percentage of 50 %. Antibiotics sensitivity test revealed that Enrofloxacin is the most effective antibiotic against Salmonella Enteritidis . On the other hand, the obtained isolates of Salmonella Enteritidis showed resistant to Sulfisoxazole followed by Penicillin and Ampicillen and low level of resistance was found for Gentamicin, Streptomycin, Ceftriaxone and Trimethoprim/Sulfamethoxazole. In vitro assessment of the effect of β-lactamase-producing Salmonella Enteritidis on the sensitivity of Staph. aureus to penicillin. Results revealed significantly lower colony count of Staph. auerus in mixed culture with β-lactamase-producing Salmonella Enteritidis and Penicillin in comparison with control groups ,the results of the present study necessitate further exploration of genetic elements related to antibiotic resistance and underlines the need to track the evolution of β-lactamases in Salmonella Entritidis isolates in Egypt.