الفهرس | Only 14 pages are availabe for public view |
Abstract Fruit processing produces large amounts of waste, which is in the peels and seeds, etc. Its disposal represents a big problem that required direction to exploit these waste uses more useful, such as use as additives for food or feed supplement because they contain a high nutritional value of earning great importance. Also headed low profile to be used in the treatment earning them a great economic importance, since it is well known that these products represent an important source of sugars, minerals and organic acids, in addition to dietary fiber and polyphenols which have many adverse effects of cancers, viruses and bacteria and heart disease, the research aims to evaluate the possibility of eating products secondary resulting from the manufacture of certain fruits such as grapes (seeds), oranges (peels) as a source of high-value compounds in the treatment of cancers and viruses .In recent years, significant progress in the development of new antiviral drugs and cancer event It was found that natural products and plant compounds derived (phytochemicals), are important for the pharmaceutical anti-viral. The potential sources raised the inadequacy of the current methods for the treatment of cancer search for new strategies, which are more effective and have a greater acceptance patient is due to the high toxicity associated current treatment for cancer system, an increasing number of patients resort to complementary therapies, which often involve the use of natural products, phytochemicals specifically, given the widespread use of these products and complementary therapies in many diseases, including cancer, it has become it is necessary to characterize these compounds scientifically and understand the mechanism of action. The grape seeds from plant products which are used as feed supplement extract. The effectiveness of grape seed extract has been shown as an antidote to many types of cancers such as lung, breast and colon, as recommended by some epidemiological studies that diets containing Alvitusterol of orange peel (albedo) reduces the risk of colon cancer and breast cancer in humans This study developed in order to assess the activity of grape seeds and orange peel extracts albedo as anti-virus and cancer cells and the effect of these extracts on the genes responsible for the death of cancer cells So it was the work of Ithanolic and Mithanolic extracts from both grape seeds and orange peels albedo and test the effect of each of them to the cancer cells such as breast and colon cells, and to identify deadly for fifty percent of the cells focus using dye : methyl Thiazulal diphenyl Ttrasulam bromide (MTT) .As it has been addressed breast cancer and colon cancer cell, extracts tested for 24 hours and examined under the microscope to determine the inverted cellular morphological changes. As well as study the effect of different concentrations of the extracts tested the genes responsible for programmed cell death using the polymerase chain reaction to genes: P – 53, Bax, BCL -2. The effect of tested extracts evaluated as materials retardant viruses Rift Valley Fever virus and hepatitis A virus , by identifying the security focus of these extracts on cells Vero using the MTT dye, has been used these concentrations in the study of the impact damper of these extracts on viruses tested by identifying the rate of decline the concentration of the virus after treatment Vero cells visits extracts tested positive for the virus before and again after injury and compared with a concentration of the virus to cells untreated extracts tested.biological potential of the extracts. You should achieve higher level of characterization of the contents of the extracts and evaluated to target the most promising parts of which have a higher biological activity. |