الفهرس 
ABSTRACTOnly 14 pages are availabe for public view
 |  | from | 222 |  |  |
| | | from | 222 | | |
Abstract
The study objective was to prepare 2 vaccines of fowl cholera 1st one is bivalent inactivated Fowl Cholera (P.multocida (5:A, D2)) vaccine with Montanide ISA70 oil adjuvant (1). 2nd one bivalent inactivated Fowl Cholera (P.multocida (5: a & D2) vaccine with white oil adjuvant (2).Each vaccine (1) & (2) was vaccinated in a group of chickens (4 weeks old) with dose 0.5 ml S/C, booster dose was given after 1stdose with4 weeks (chicken 8 weeks old) in the 2 groups. Regular withdrawal of blood samples every week. Applying of PHA test on serum collected from 2groups chicken it was clear that AB titer from vaccine (1) is higher than AB titer in group (2). Applying challenge test on the 2 groups of vaccinated chickens in vaccine (1) protection % for 5: a is 95% & D2 is 90%, while for vaccine (2) 5: a is 90%&& D2 is 85%.
Depending on these results from the previous experiments, 2vaccinal batches were prepared using Montanide ISA 70 oil adjuvant in the 2 vaccine. 1st one (Bivalent inactivated AI vaccine) (3). 2nd one (inactivated combined (AI + FC) vaccine (4). each batch was vaccinated in a group of chicken 4weeks old, o.5ml S/C one single dose (no booster dose).HI test was applied on serum collected from vaccinated chickens regularly each week. The result showed AB titer for S1 strain is higher than AB titer in S2 Strain in both vaccines. By applying challenge test using local virulent HPAI (H5N1) challenge virus, the protection % in group (3) 93.3%& in group (4) 86.6%