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العنوان
Toxicological effects of nanoparticles on the
common house mosquito, Culex pipiens L.
(Diptera: Culicidae) /
المؤلف
Elsebaey,Ibrahim Rabie Abd El-aziz.
هيئة الاعداد
باحث / Ibrahim Rabie Abd El-aziz Elsebaey
مشرف / Reda Fadeel Ali Bakr
مشرف / Noha Awny Mohamed Guneidy
مشرف / Mohamed Said Attia
تاريخ النشر
2016
عدد الصفحات
132p.;
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم الحشرات
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة عين شمس - كلية العلوم - علم الحشرات
الفهرس
Only 14 pages are availabe for public view

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Abstract

The present study aimed to evaluate the efficiency of two
types of nanoparticles against 3rd instar larvae of Cx. pipiens. This
study was divided into three main parts:
1. The first part included the synthesis of the nanoparticles
and their characterization using transmission electron
microscope (TEM).
2. The second part included the evaluation of the efficiency
of synthesized nanoparticles against the target vector
species.
3. The third part included the investigation of the
biochemical impacts of the treatment of mosquito larvae
with the synthesized nanoparticles.
1- Synthesis of nanoparticles:
A) Silica nanoparticles:
Silica nanoparticles were synthesized via a sol-gel method to
obtain monodispersed nanoparticles. Transmission electron
microscope TEM micrographs showed that the most
nanoparticles in the sample were monodispersed with an average
particle size below 10 nm. Particles with size of 3.15, 2.83, 3.33,
4.33, 5.92 and 6.06 nm were observed by TEM image analysis
software. Simple degree of agglomeration of the particles was
noticed in the sample. The properties of the obtained
nanoparticles were satisfactorily controlled.
B) Hematite nanoparticles:
Hematite nanoparticles were synthesized via a hypothermal
method to obtain magnetic nanoparticles. TEM micrographs
showed that the most nanoparticles in the sample were
agglomerated with an average particle size below 10 nm.
Particles with size of 5.21, 5.24, 5.42, 5.86, 8.43 and 9.12 nm
were observed by TEM image analysis software. Agglomeration
of nanoparticles was noticed in the sample.
2- Toxicological Studies:
2.1 Toxicity of nanoparticles against 3rd instar larvae of Cx.
pipiens:
The obtained results indicated that the toxicity of tested
nanoparticles varied according to the concentration used, and the
chemical structure. Data revealed that LC50 estimates on the 3rd
instar larvae of Cx. pipiens was 0.6662 and 5.7036 ppm for SNPs
and HNPs, respectively. While, LC90 estimates were 17.2472 and
648.7061 ppm for same nanoparticles, respectively.
Toxicological evaluation revealed that SNPs were the most
potent against the 3rd instar larvae of Cx. pipiens.
2.2 Effect of time variation on the larvicidal activity of tested
nanoparticles against 3rd instar larvae of Cx. pipiens:
In case of SNPs, LT50 estimates corresponding to the tested
concentrations (0.025, 0.25, 2.5 and 25 ppm) were 143.23, 39.81,
11.73 and 7.86 hrs. While, LT50 estimates corresponding to the
tested concentrations of HNPs (0.025, 0.25, 2.5 and 25 ppm) were
125.49, 76.12, 74.68 and 36.11 hrs. The obtained results at
concentration (25 ppm) for the tested nanoparticles indicated that
SNPs were the most efficient and the fastest in action.
3- Biochemical studies:
3.1 Determination of total protein and lipid of untreated and
treated 3rd instar larvae of Cx. pipiens:
Treatment with LC25 and LC50 of HNPs and SNPs reduced the
total lipid content with values of 2.49, 3.83, 7.09 and 27.549 %,
respectively. Treatment with LC25 and LC50 of HNPs increased
total protein content with values of 1.96 and 28.02%,
respectively. Treatment with LC25 and LC50 of SNPs obtained
induced significant reduction in total protein content of treated
larvae with values of 9.71 and 13.37%, respectively.
3.2 Electrophoretic fraction protein patterns (SDS-PAGE)
of untreated and treated 3rd instar larvae of Cx. pipiens:
The fraction patterns of larval proteins showed different
numbers of protein bands according to their molecular weights,
they were separated into 31 rows. Electrophoresis revealed
differences between the untreated and treated samples. The
average bands number of treated samples ranged between 8-13
bands comparing with 8 bands for untreated control. Bands
appeared in untreated sample were with molecular weights
ranged between (250 and 5) kDa. The total number of bands in
sample 2 (treated with LC25 of HNPs) were 8 bands appeared
with molecular weights ranged between (140.2 and 11.169) kDa.
The total number of bands in sample 3 (treated with LC50 of
HNPs) were 12 bands appeared with molecular weights ranged
between (250 and 10.765) kDa. The total number of bands in
sample 4 (treated with LC25 of SNPs) were 8 bands appeared with
molecular weights ranged between (105.2 and 10.765) kDa. The
total number of bands in sample 5 (treated with LC50 of SNPs)
were 13 bands appeared molecular weights ranged between (250
and 10.765) kDa.