الفهرس 
1.ObesityOnly 14 pages are availabe for public view
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Abstract
T
his study was carried out at Medical Research Centre, Faculty of Medicine, Ain Shams University in the period from March 2013 till June 2014 and included 30 obese subjects and 30 non-obese controls.
The aim of this study was to determine the changes of miRNA-143 expression in obesity compared to normal individuals.
The studied individuals were divided into three main groups:
Group I: Healthy normal individuals with BMI between 18.5 to 24.9 (n=15, mean BMI=24±1 and mean age=44±11).
Group II: Overweight individuals with BMI between 25 to 29.9 (n=15, mean BMI=27.8±1 and mean age=38±10).
Group III: Obese individuals with BMI 30 or more (n=30, mean BMI=42±11 and mean age=37±11).
All participants of this study went through complete medical assessment including family history of chronic non-communicable diseases, pattern of physical activity, symptoms covering various systems and general examination including blood pressure measurements were done.
Furthermore, anthropometric measurements were performed for them. BMI was calculated as the ratio of the weight and height squared (kg/m2) and assessment of BMI was done using categories reported by WHO (2012). In addition, laboratory investigations including fasting blood sugar and complete lipid profile were done.
Finally, miRNA extraction from serum and adipose tissue of all subjects was done. Reverse transcription of miR-143 and comparative quantitation of its expression by using Real-Time PCR were done for all samples. Then, the collected data were statistically analyzed by the SPSS software.
The results of this study showed that there was high expression of miR-143 in paired serum and adipose tissue samples of the obese group compared to normal and overweight groups indicating its upregulation in human obesity. There was a highly significant positive correlation between fold change of miRNA-143 expression in serum and BMI among the three groups as well as a positive correlation between its expression in tissue and BMI among them.
There was no significant correlation between miR-143 expression and any of the demographic data of the patients assuming that it may be an independent biomarker and therapeutic target in obesity.