الفهرس 
الملخص العربىOnly 14 pages are availabe for public view
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Abstract
The aim of this study is to investigate the usage of magnetite nanoparticles MNPs)to reduce the cytotoxicity and to enhance the cellular uptake of the nanoparticles. In this study, the prepared magnetite nanoparticles and magnetite core shell with gold have been characterized by TEM and vibrating sample magnetometer (VSM) . The effect of cellular uptake of magnetite and gold core shell on cell adhesion/viability, cytotoxicity and morphology of colon cancer cells culture (HCT16) have been investigated.Cell cytotoxicity/adhesion of MNPs when injected in HCT16 has been studied. The study showed that the magnetic nanoparticles are non-toxic and displayed excellent in vitro biocompatibility. In vitro MRI measurements show significantly reduced water proton relaxation times of both T1 and T2 The magnetite core shells with gold nanoparticles (Fe3O4-Au) are found to be non-toxic while HCT16 colon cancer cells incubated with different concentrations of the Fe3O4-Au nanoparticles exposed to light emitted diode at 530 nm and power of 250 mW, showed a remarkable effect on cell viability.Transmission electron microscopy results indicated that the MNPs were internalised into cells via different mechanisms. This is due to that the nanoparticles internalize the cell via endocytosis and exocytose behavior depending on the incubation time of the nanoparticles.To the best of our knowledge, this is the first time to use laser-induced breakdown spectroscopy technique (LIBS) for identification of the effect of metals magnetic nanoparticles at different concentrations on colon cancer cell (HCT16 cell) uptake. Our results indicate that increasing concentration of iron oxide nanoparticles is associated with increasing concentration of calcium in the test colon cancer cell line. Finally LIBS has been demonstrated successfully as an effective technique for the determination of magnetic nanoparticles concentrations present in colon cancer cell.