الفهرس 
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Abstract
In this study bacteriological examination of 217 fecal samples (145 fecal samples were taken from the rectum of diarrheic horses and foal , and 72 fecal samples from apparently healthy horses and foals) in addition to 69 Vaginal swabs and 24 double Guarded uterine swabs were collected from mares suffering of repeat breeder (infertility and miscarriage), and 8 swabs were taken aseptically from the stomach contents of the aborted fetuses revealed isolation of 8 Salmonella isolates with an overall incidence 2.52%. It was notice that out of 145 horses suffering of diarrhea 3 Salmonella isolates were recovered with a recovery rate 2.07 %, Regarding to 93 genital swabs collected from horses had infertility problems 4 samples were positive with a recovery rate 4.3%, moreover one out of 8 aborted fetuses was positive for Salmonella with a recovery rate 12.5%, while no Salmonella isolates could be recovered from apparently healthy horses. Serological identification of Salmonella revealed isolation of Salmonella Newport and Salmonella Kentuky with an incidence of 1.14% for each, while one isolate of Salmonella Typhimurium was recovered from 57 foals had diarrhea with an incidence 1.75%. In the present study, it was observed that the most recurrent serovar was S.Typhimurium which was isolated from 3 vaginal swabs of horses had infertility problem with an incidence of 4.35%. On the other hand S. Abortus equi could be isolated from horse suffering of infertility (repeat breeder) with an incidence of 4.12% and one isolates of S.Abortus equi could be recovered from stomach content of aborted 2 fetus. A total number of 8 Salmonella isolates were investigated for the presence of virulence genes revealed that all isolates harbor invA gene and amplified 284 bp fragments. In the present investigation we studied the location of pef gene plasmid encoded fimbriae and stn gene Salmonella enterotoxin in total DNA or plasmid by using PCR. One isolate of S.Typhimurium (vaginal swab) detected harbor both pef and stn genes on the total DNA. The stn gene was encoded on plasmid DNA and amplified a region 600 bp in all isolates, While four isolates of Salmonella (S. Typhimurium (3) and S.Kentuky(1) possess pef gene and detected by the presence of 700 bp product that encoded in the plasmid DNA. We can concluded that the stn and pef genes were encoded mainly on plasmid DNA and hence on total DNA.